| Info |
|---|
| Info |
Plates in red have been extracted, PCRed, cleaned, quantified, and normalized by 7-15-22. Bolded red have been qPCRed. |
NS6 Plates
6AC1 | 6AC2 | 6AC3 | |
6BM1 | 6BM2 | ||
6LVD1 | 6LVD2 | 6LVD3 | 6LVD4 |
6RD1 | 6RD2 | 6RD3 | |
6GTL1 | 6GTL2 | ||
6RD4 | 6RD5 | 6RD6 | 6RD7 |
6LVD5 |
|
| |
MP002 | MP003 | MP006 |
MasterMix (make for 16 plates in 50mL conical tube)
...
Add 16 ul of Illumina Library Quantification MasterMix to each well:
1
2
3
4
5
6
7
8
9
10
11
12
A
6AC3_ITS
6AC3_16S
6RD1_ITS
6RD1_16S
6BM1_ITS
6BM1_16S
6LVD1_ITS
6LVD1_16S
6GTL1_ITS
6GTL1_16S
20 pM Std
20 pM Std
B
6AC3_ITS
6AC3_16S
6RD1_ITS
6RD1_16S
6BM1_ITS
6BM1_16S
6LVD1_ITS
6LVD1_16S
6GTL1_ITS
6GTL1_16S
2 pM Std
2 pM Std
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
10 ul | KAPA SYBR FAST qPCR MM (2X) | 110 | 1100 |
2 ul | Primer Premix (10X) | 110 | 220 |
4 ul | Ultra Pure Water | 110 | 440 |
16 ul | Total Volume | 110 | 1760 |
Add 4 ul of template, pool, or standards to each well:
110 | 220 | ||
4 ul | Ultra Pure Water | 110 | 440 |
16 ul | Total Volume | 110 | 1760 |
Add 4 ul of template, pool, or standards to each well:
Plate Name
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | 0.2 20 pM Std0.2 | 20 pM Std | ||
DB | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | 0.02 2 pM Std0.02 | 2 pM Std | ||
EC | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | 0.002 2 pM Std | 0.002 2 pM Std | ||
FD | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | 0.0002 02 pM Std | 0.0002 02 pM Std | ||
GE | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | NTC | NTC | H0.002 pM Std | 0.002 pM Std |
F | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | NTC | NTC |
Results:
0.0002 pM Std | 0.0002 pM Std |
G | 6AC3_ITS |
4.125
6AC3_16S |
6RD1_ITS |
10.152
6RD1_16S |
15.709
6BM1_ITS |
7.874
6BM1_16S |
6LVD1_ITS |
4.532
6LVD1_16S |
6GTL1_ITS |
5.992
6GTL1_16S |
Needs to be redone
Full qPCR results below:
| View file | ||
|---|---|---|
|
qPCR NovaSeq6_Set3
Make 1:1000 dilutions of column 8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
1
2
3
4
5
6
7
8
9
10
11
12
A
6LVD2_ITS
6LVD2_16S
2TRNL3_16S
2TRNL3_TRNL
2TRNL2_TRNL
2TRNL2_16S
6RD2_16S
6RD2_ITS
6GTL1_16S
2TRNL1_16S_2
NTC
B
6LVD2_ITS
6LVD2_16S
2TRNL3_16S
2TRNL3_TRNL
2TRNL2_TRNL
2TRNL2_16S
6RD2_16S
6RD2_ITS
6GTL1_16S
2TRNL1_16S_2
04.0002 pM Std
0.0002 pM Std
C
6LVD2_ITS
6LVD2_16S
2TRNL3_16S
2TRNL3_TRNL
2TRNL2_TRNL
2TRNL2_16S
6RD2_16S
6RD2_ITS
6GTL1_16S
2TRNL1_16S_2
0.002 pM Std
0.002 pM Std
NTC | NTC | |||||||||||
H | 6AC3_ITS | 6AC3_16S | 6RD1_ITS | 6RD1_16S | 6BM1_ITS | 6BM1_16S | 6LVD1_ITS | 6LVD1_16S | 6GTL1_ITS | 6GTL1_16S | NTC | NTC |
Results:
Plate Name | Average Result (nanomoles) |
|---|---|
6AC3_ITS | 4.125 |
6AC3_16S | 7.46 |
6RD1_ITS | 10.152 |
6RD1_16S | 15.709 |
6BM1_ITS | 7.874 |
6BM1_16S | 4.842 |
6LVD1_ITS | 4.532 |
6LVD1_16S | 8.102 |
6GTL1_ITS | 5.992 |
6GTL1_16S | Needs to be redone |
Full qPCR results below:
| View file | ||
|---|---|---|
|
qPCR NovaSeq6_Set3
Make 1:1000 dilutions of column 8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
Add 4 ul of template, pool, or standards to each well:
1
2
3
4
5
6
7
8
9
10
11
12
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 0.02 pM Std | 0.02 pM Std | E | NTC |
B | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 004.2 0002 pM Std | 0.2 0002 pM Std | ||
FC | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 2 0.002 pM Std | 2 0.002 pM Std | ||
GD | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 20 0.02 pM Std | 20 0.02 pM Std | ||
HE | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S2TRNL1_16S_2 |
|
|
Add 16 ul of Illumina Library Quantification MasterMix to each well:
...
ul/rxn
...
Reagent
...
# of rxns
...
ul needed
...
10 ul
...
KAPA SYBR FAST qPCR MM (2X)
...
110
...
1100
...
2 ul
...
Primer Premix (10X)
...
110
...
220
...
4 ul
...
Ultra Pure Water
...
110
...
440
...
16 ul
...
Total Volume
...
110
...
1760
2TRNL1_16S_2 | 0.2 pM Std | 0.2 pM Std | ||||||||||
F | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 2 pM Std | 2 pM Std |
G | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 20 pM Std | 20 pM Std |
H | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S |
2 pM Std
2 pM Std
C
6LVD2_ITS
6LVD2_16S
2TRNL3_16S
2TRNL3_TRNL
2TRNL2_TRNL
2TRNL2_16S
6RD2_16S
6RD2_ITS
6GTL1_16S
2TRNL1_16S_2
0.2 pM Std
0.2 pM Std
_2 |
|
|
Add 16 ul of Illumina Library Quantification MasterMix to each well:
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
10 ul | KAPA SYBR FAST qPCR MM (2X) | 110 | 1100 |
2 ul | Primer Premix (10X) | 110 | 220 |
4 ul | Ultra Pure Water | 110 | 440 |
16 ul | Total Volume | 110 | 1760 |
Add 4 ul of template, pool, or standards to each well:
Plate Name
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 0.02 20 pM Std0.02 | 20 pM Std | ||
EB | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 0.002 2 pM Std0.002 | 2 pM Std | ||
FC | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | 0.0002 2 pM Std | 0.0002 2 pM Std | ||
GD | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | NTC | NTC | H0.02 pM Std | 0.02 pM Std |
E | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | NTC | NTC |
Results:
0.002 pM Std | 0.002 pM Std |
F | 6LVD2_ITS |
16.09
6LVD2_16S |
2TRNL3_16S |
2TRNL3_TRNL |
2TRNL2_TRNL |
2TRNL2_16S |
6RD2_16S |
28.48
6RD2_ITS |
29.45
6GTL1_16S |
2TRNL1_16S_2 |
0. |
Full qPCR results below:
...
0002 pM Std | 0.0002 pM Std | ||||
G | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_ |
...
qPCR NovaSeq6_Set4
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
...
...
1
...
2
...
3
...
4
...
5
...
6
...
7
...
8
...
9
...
10
...
11
...
12
...
A
...
...
...
NTC
...
B
...
...
04.0002 pM Std
...
0.0002 pM Std
...
C
...
...
0.002 pM Std
...
0.002 pM Std
...
D
...
...
0.02 pM Std
...
0.02 pM Std
...
E
...
...
0.2 pM Std
...
0.2 pM Std
...
F
...
...
2 pM Std
...
2 pM Std
...
G
...
...
20 pM Std
...
20 pM Std
...
H
...
...
...
Add 16 ul of Illumina Library Quantification MasterMix to each well:
...
ul/rxn
...
Reagent
...
# of rxns
...
ul needed
...
10 ul
...
KAPA SYBR FAST qPCR MM (2X)
...
110
...
1100
...
2 ul
...
Primer Premix (10X)
...
110
...
220
...
4 ul
...
Ultra Pure Water
...
110
...
440
...
16 ul
...
Total Volume
...
110
...
1760
Add 4 ul of template, pool, or standards to each well:
1
2
3
4
5
6
7
8
9
10
11
12
A
NTC
NTC
NTC
B
TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | NTC | NTC | |||||
H | 6LVD2_ITS | 6LVD2_16S | 2TRNL3_16S | 2TRNL3_TRNL | 2TRNL2_TRNL | 2TRNL2_16S | 6RD2_16S | 6RD2_ITS | 6GTL1_16S | 2TRNL1_16S_2 | NTC | NTC |
Results:
Plate Name | Average Result (nanomoles) |
|---|---|
6LVD2_ITS | 16.09 |
6LVD2_16S | 21.71 |
2TRNL3_16S | 42.69 |
2TRNL3_TRNL | 8.68 |
2TRNL2_TRNL | 7.51 |
2TRNL2_16S | 36.27 |
6RD2_16S | 28.48 |
6RD2_ITS | 29.45 |
6GTL1_16S | 8.2 |
2TRNL1_16S_2 | 32.72 |
Full qPCR results below:
| View file | ||
|---|---|---|
|
qPCR NovaSeq6_Set4
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_16S_A1 |
|
| NTC |
B | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_16S_B1 |
| 04.0002 pM Std | 0.0002 pM Std |
0.0002 pM Std
C
C | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_16S_C1 |
| 0.002 pM Std | 0.002 pM Std |
D |
0.02 pM Std
0.02 pM Std
0.02 pM Std
E
0.2 pM Std
6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_16S_D1 |
| 0.02 pM Std | 0. |
Full qPCR results below:
qPCR NovaSeq6_Set5
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
1
2
3
4
5
6
7
8
9
10
11
12
A
NTC
B
04.0002 pM Std
0.0002 pM Std
C
0.002 pM Std
0.002 pM Std
D
0.02 pM Std
0.02 pM Std
E
0.2 pM Std
0.2 pM Std
F
2 pM Std
2 pM Std
G
20 pM Std
20 pM Std
H
02 pM Std |
F
2 pM Std
2 pM Std
2 pM Std
G
20 pM Std
20 pM Std
20 pM Std
H
Results:
...
Plate Name
...
Average Result (nanomoles)
E | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_ITS_A8 |
| 0.2 pM Std | 0.2 pM Std |
F | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_ITS_B8 |
| 2 pM Std | 2 pM Std |
G | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_ITS_C8 |
| 20 pM Std | 20 pM Std |
H | 6RD4_16S_Col3 | 6RD4_ITS_Col3 | 6RD5_16S_Col3 | 6RD5_ITS_Col3 | 6RD6_16S_Col3 | 6RD6_ITS_Col3 | 6RD7_16S_Col3 | 6RD7_ITS_Col3 | 6LVD5_ITS_D8 |
|
|
|
Add 16 ul of Illumina Library Quantification MasterMix to each well:
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
10 ul | KAPA SYBR FAST qPCR MM (2X) | 110 | 1100 |
2 ul | Primer Premix (10X) | 110 | 220 |
4 ul | Ultra Pure Water | 110 | 440 |
16 ul | Total Volume | 110 | 1760 |
Add 4 ul of template, pool, or standards to each well:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|
11
12
A
NTC
NTC
NTC
B
0.0002 pM Std
0.0002 pM Std
0.0002 pM Std
C
0.002 pM Std
0.002 pM Std
0.002 pM Std
D
0.02 pM Std
0.02 pM Std
0.02 pM Std
E
0.2 pM Std
0.2 pM Std
0.2 pM Std
F
2 pM Std
2 pM Std
2 pM Std
G
20 pM Std
20 pM Std
20 pM Std
H
Results:
...
Plate Name
...
Average Result (nanomoles)
Full qPCR results below:
qPCR NovaSeq6_Set6
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
...
G
...
20 pM Std
...
20 pM Std
...
20 pM Std
...
H
...
...
...
Results:
...
Plate Name
...
Average Result (nanomoles)
...
1
2
3
4
5
6
7
8
9
10
11
12
A
NTC
B
04.0002 pM Std
0.0002 pM Std
C
0.002 pM Std
0.002 pM Std
D
11 | 12 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_16S | NTC | NTC | NTC |
B | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_16S | 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_16S | 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_16S | 0.02 pM Std | 0.02 pM Std | 0.02 pM Std |
E |
0.2 pM Std
0.2 pM Std
F
6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_ITS | 0.2 pM Std | 0.2 pM Std |
G
0.2 pM Std |
20 pM Std
H
Add 16 ul of Illumina Library Quantification MasterMix to each well:
...
ul/rxn
...
Reagent
...
# of rxns
...
ul needed
...
10 ul
...
KAPA SYBR FAST qPCR MM (2X)
...
110
...
1100
...
2 ul
...
Primer Premix (10X)
...
110
...
220
...
4 ul
...
Ultra Pure Water
...
110
...
440
...
16 ul
...
Total Volume
...
110
...
1760
Add 4 ul of template, pool, or standards to each well:
...
...
1
...
2
...
3
...
4
...
5
...
6
...
7
...
8
...
9
...
10
...
11
...
12
...
A
...
NTC
...
NTC
...
NTC
...
B
...
0.0002 pM Std
...
0.0002 pM Std
...
0.0002 pM Std
...
C
...
0.002 pM Std
...
0.002 pM Std
...
0.002 pM Std
...
D
...
0.02 pM Std
...
0.02 pM Std
...
0.02 pM Std
...
E
...
0.2 pM Std
...
0.2 pM Std
...
0.2 pM Std
...
F
...
2 pM Std
...
2 pM Std
...
2 pM Std
F | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_ITS | 2 pM Std | 2 pM Std | 2 pM Std |
G | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_ITS | 20 pM Std | 20 pM Std | 20 pM Std |
H | 6RD4_16S | 6RD4_ITS | 6RD5_16S | 6RD5_ITS | 6RD6_16S | 6RD6_ITS | 6RD7_16S | 6RD7_ITS | 6LVD5_ITS | NS6_Pool_1:1000 | NS6_Pool_1:1000 | NS6_Pool_1:1000 |
Results:
Plate Name | Average Result (nanomoles) |
|---|---|
6RD4_16S | 70.66 |
6RD4_ITS | 22.2 |
6RD5_16S | 76.68 |
6RD5_ITS | 86.82 |
6RD6_16S | 32.57 |
6RD6_ITS | 45.66 |
6RD7_16S | 45.71 |
6RD7_ITS | 67.98 |
6LVD5_16S | 50.03 |
6LVD5_ITS | 14.98 |
NS6_Pool | 12.17 |
Full qPCR results below:
| View file | ||
|---|---|---|
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