...
Average results for the following plates (column 3):
4MR1_16S: 92.23 nanomoles
4MR1_ITS: 53.47 nanomoles
4MR2_16S: 105.34 nanomoles
4MR2_ITS: 66.71 nanomoles
4SC1_16S: 46.64 nanomoles
4SC1_ITS: 47.77 nanomoles
4SC2_16S: 65.97 nanomoles
4SC2_ITS: 55.69 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|
MasterMix (make for 24 plates in 50mL conical tube)
...
Average results for the following plates (column 3):
4LVD1_16S: 112.54 nanomoles
4LVD1_ITS: 24.71 nanomoles
4LVD2_16S: 95.57 nanomoles
4LVD2_ITS: 34.28 nanomoles
4LVD3_16S: 112.47 nanomoles
4LVD3_ITS: 101.03 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|
qPCR NovaSeq4_Plate5
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
Use other half of NS4_Plate 4 dilution plate to do NS4_Plate5 dilution plate.
...
Average results for the following plates (column 3):
4FB1_16S: 28.57 nanomoles
4FB1_ITS: 45.42 nanomoles
4FB2_16S: 70.82 nanomoles
4FB2_ITS: 71.52 nanomoles
4FB3_16S: 73.04 nanomoles
4FB3_ITS: 72.32 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|