| Info |
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4-06-2022 10-21-2022 Contamination has been detected in most blanks likely from an Alfalfa 16S/ITS project that bookended this one. Many plates will need to be rerun. 5-02-2022 Data have been processed and can be found in (/project/microbiome/data/seq/cu_29april22novaseq5) on Teton. Data were returned to us 4-29-22. Report confusion has been cleared up. It involved using the index reads they ran in their calculations. All samples have been pooled and should be were mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus normalization protocol, Normalization_Tracking_Adaptive to 10 ng/ul, leaving samples below this as is. We normalized in 20 ul, unless the starting concentration was above 100 ng/ul. We then normalized in 50 ul for those samples only. 2 ul from each sample was added to a new plate and then this plate was pooled into a column and the column was pooled into a 15 ml tube. |
Flowcell Summary
Clusters (Raw) | Clusters(PF) | Yield (MBases) |
|---|---|---|
985,502,477 | 956,877,735 | 494,722 |
Lane Summary
Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|
1 | 492,110,025 | 100.00 | 100.00 | NaN | 247,039 | 77.09 | 87.48 | 34.69 |
2 | 493,392,452 | 100.00 | 100.00 | NaN | 247,683 | 77.29 | 87.47 | 34.69 |
Include Yoon plate for resequencing(16S0C8, 16S0D8, ITS0C8, ITS0D8). This just needs to be added to the pool.
In MISO | Researcher | GTL Plate Name | |||||||
|---|---|---|---|---|---|---|---|---|---|
X | Felix B. | 5FB1 | 5FB2 | 5FB3 | 5FB4 | 5FB5 | |||
X | Ayda L. | 5AL1 | 5AL2 | 5AL3 | 5AL4 | 5AL5 | 5AL6 | 5AL7 | 5AL8 |
X | Linda VD | 5LVD1 | 5LVD2 | 5LVD3 | 5LVD4 | 5LVD5 | |||
X | Dulcinea G. | 5DG1 | 5DG2 | 5DG3 | 5DG4 | 5DG5 | |||
X | R. Dibner | ||||||||