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4-2810-21-2022 Contamination has been detected in most blanks likely from an Alfalfa 16S/ITS project that bookended this one. Many plates will need to be rerun. 5-02-2022 Data have been processed and can be found in (/project/microbiome/data/seq/cu_29april22novaseq5) on Teton. Data were returned to us . There is some confusion over the provided percent clusters passing filter in comparison with past runs, but I am proceeding with processing for now4-29-22. Report confusion has been cleared up. It involved using the index reads they ran in their calculations. All samples have been pooled and were mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus normalization protocol, Normalization_Tracking_Adaptive to 10 ng/ul, leaving samples below this as is. We normalized in 20 ul, unless the starting concentration was above 100 ng/ul. We then normalized in 50 ul for those samples only. 2 ul from each sample was added to a new plate and then this plate was pooled into a column and the column was pooled into a 15 ml tube. |
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