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Status: the library prep has been completed and shipped to USU. We need to invoice USU for the preps. |
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This was the page for a first attempt at making this library and is superseded by its parent page.
Quantify DNAs on Synergy HTX Plate Reader:
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Normalize DNAs on Nimbus:
Had to define these semi skirted plates for the Nimbus. We might want to standardize to full skirted plates. They are less variable.
16S Library Prep:
Swap out KG4 G9’s blank for Mock Community positive control
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Temp C | Cycles | Time |
|---|---|---|
95* | 1X | 3:00 |
98 | 19X | 0:30 |
55* | 19X | 0:30 |
72 | 19X | 0:30 |
72 | 1X | 5:00 |
4 | 1X | 0:00 |
Purify samples using GSAF’s second modified MagBead protocol:
Equilibrate Beads to room Temperature
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____________________________________________________________________________________________________________
ITS Library Prep:
Swap out KG4 G9’s blank for Mock Community positive control
...
Temp C | Cycles | Time |
|---|---|---|
95* | 1X | 3:00 |
98 | 19X | 0:30 |
55* | 19X | 0:30 |
72 | 19X | 0:30 |
72 | 1X | 5:00 |
4 | 1X | 0:00 |
Purify samples using GSAF’s second modified MagBead protocol:
Equilibrate Beads to room Temperature
...