| Info |
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New aliquots of the 4 pools were mailed to the University of Colorado on 10/13/2021 for deeper sequencing. Data was retrieved on 11/09/2021 The four pools were mailed to the University of Colorado’s Genomics Core on 03/23/2021. Pools were ran on 03/30/21. Data uploaded 4/02/2021. |
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Lane Summary (Pools 1 and 2) AHNYYMDRXY
Pool | Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|---|
1 | 1 | 963,211,753 | 100 | 100 | NaN | 104,990 | 75.45 | 89.09 | 34.84 |
2 | 2 | 926,591,105 | 100 | 100 | NaN | 100,998 | 72.58 | 89.23 | 34.86 |
Lane Summary (Pools 3 and 4) BHNYW7DRXY
Pool | Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|---|
3 | 1 | 972,876,446 | 100 | 100 | NaN | 106,044 | 76.2 | 89.69 | 34.95 |
4 | 2 | 918,555,590 | 100 | 100 | NaN | 100,123 | 71.95 | 88.79 | 34.79 |
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Pool | Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|---|
3 | 1 | 482,619,189 | 100.00 | 100.00 | NaN | 52,605 | 75.61 | 89.39 | 34.89 |
4 | 2 | 427,134,565 | 100.00 | 100.00 | NaN | 46,558 | 66.91 | 87.90 | 34.62 |
Reagents and Ordering
- EcoR1 (20,000 units/ml)
- MseI (10,000 units/ml)
- T4 DNA ligase buffer (400,000 units/ml)
- DNA polymerase (BioRad iProof or KAPA HiFi)
- BSA (1 mg/ml)-We have this in 20 mg/ul
- 1 M NaCl-We have this at 5M
- DMSO
- EcoR1 adaptors (We need 1 uM MIDed in plates)
- Mse1 Adaptors (10 uM stock probably need to dilute from 100 uM)
- IIllpcr2 (10 uM working)
- Illpcr1 (10 uM working)
- Pool of above 2 primers (5 uM working of each)
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