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Add 11 ul to each well of a hard shell, full skirt plate. Seal with bubble strips and store in refrigerator until needed label “NS6 PCR”. (If doing manually, one needs 2 ul of template and 2 ul of the primers).
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 500 | 1500 |
0.45 | 10M dNTPs | 500 | 225 |
0.3 | Kapa HiFi HotStart DNA Pol | 500 | 150 |
7.25 | HPLC H2O | 500 | 3625 |
11 | Total Volume | 500 | 5500 |
Run on Thermocycler Program GSAF36 or AMF35:
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