Setup Notes
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Normalize plate reader with Buffer AE for all plates
Quantify all plates
Check for samples above 150 ng/ul
Transfer 20 ul from all wells of a plate needing normalization to new VWR plate
Add 20 ul TE to all wells above 150 ng/ul
Restriction Digestion
(Keep MM and reaction plates on ice)
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Template | EcoR1 MID plate | Pool Plate | Library | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
WRosenthal_Trout_plate9plate12 | 1 | 2TroutRedoPool1 | 2TroutRedoPool51and2 | ||||||||||||
WRosenthal_Trout_plate10plate16 | 2 | 12TroutRedoPool1 | 2TroutRedoPool81and2 | ||||||||||||
WRosenthal_Trout_plate11plate18 | 3 | 12TroutRedoPool2 | 2TroutRedoPool91and2 | ||||||||||||
WRosenthalWrosenthal_PM_TroutYCT_plate12plate2 | 4 | 12TroutRedoPool2 | 2TroutRedoPool11and2 | ||||||||||||
WRosenthalMT_YCT_TroutRangewideRADs_plate13plate01 | 5 | 22TroutRedoPool3 | 2TroutRedoPool83and4 | ||||||||||||
WRosenthalWrosenthal_GY21_TroutYCT_plate14tray6 | 6 | 22TroutRedoPool3 | 2TroutRedoPool53and4 | ||||||||||||
WRosenthalWY_YCT_Trout2021_plate15plate14 | 7 | 22TroutRedoPool4 | 2TroutRedoPool143and4 | ||||||||||||
WRosenthalWrosenthal_PM_TroutYCT_plate16plate1 | 8 | 22TroutRedoPool4 | 2TroutRedoPool13and4 | ||||||||||||
WRosenthal_Trout_plate17plate9 | 1 | 32TroutRedoPool5 | 2TroutRedoPool135and6 | ||||||||||||
WRosenthal_Trout_plate18plate14 | 2 | 32TroutRedoPool5 | 2TroutRedoPool25and6 | ||||||||||||
WRosenthal_Trout_plate19plate20 | 3 | 2TroutRedoPool102TroutRedoPool6 | 5and6 | ||||||||||||
WRosenthal_Trout_plate20plate21 | 34 | 2TroutRedoPool6 | WRosenthal_Trout_plate21 | 4 | 2TroutRedoPool6 | WRosenthal_Trout_plate22 | 4 | 2TroutRedoPool145and6 | |||||||
WY_YCT_2021_plate10 | 5 | 2TroutRedoPool7 | 7and8 | ||||||||||||
WY_YCT_2021_plate13 | 6 | 2TroutRedoPool7 | 7and8 | ||||||||||||
WRosenthal_Trout_plate24plate10 | 1 | 42TroutRedoPool8 | 2TroutRedoPool137and8 | ||||||||||||
WRosenthal_Trout_plate25plate13 | 2 | 82TroutRedoPool8 | 2TroutRedoPool157and8 | ||||||||||||
MTWRosenthal_YCT_RangewideRADs_plate01 | 5 | 2TroutRedoPool3Trout_plate11 | 1 | 2TroutRedoPool9 | 10and14 | ||||||||||
MT_YCT_RangewideRADs_plate03 | 5 | 2TroutRedoPool9 | 9and12 | ||||||||||||
WY_YCT_2021_plate04plate11 | 6 | 72TroutRedoPool9 | 2TroutRedoPool119and12WY | ||||||||||||
MT_ | YCTWCT_ | 2021_plate095 | 2TroutRedoPool11 | WY_YCT_2021_plate10 | 5 | 2TroutRedoPool7plate15 | |||||||||
WRosenthal_Trout_plate19 | 3 | 2TroutRedoPool10 | 10and14 | ||||||||||||
WY_YCT_2021_plate11plate04 | 6 | WY_YCT_2021_plate13 | 6 | 2TroutRedoPool7 | 7 | 2TroutRedoPool11 | 11and13 | ||||||||
WY_YCT_2021_plate14plate09 | 5 | 72TroutRedoPool11 | 2TroutRedoPool411and13 | ||||||||||||
Wrosenthal_GY21_YCT_tray1tray3 | 7 | 2TroutRedoPool162TroutRedoPool12 | 9and12 | ||||||||||||
Wrosenthal_GY21_YCT_tray3tray7 | 78 | 2TroutRedoPool12 | Wrosenthal_GY21_YCT_tray4 | 6 | 2TroutRedoPool15 | Wrosenthal_GY21_YCT_tray6 | 6 | 2TroutRedoPool3 | Wrosenthal_PM_YCT_plate2 | 4 | 2TroutRedoPool2 | Wrosenthal_PM_YCT_plate1 | 8 | 2TroutRedoPool4 | MT_WCT_plate159and12 |
WRosenthal_Trout_plate17 | 3 | 2TroutRedoPool13 | 11and13 | ||||||||||||
WRosenthal_Trout_plate24 | 4 | 2TroutRedoPool13 | 11and13 | ||||||||||||
WRosenthal_Trout_plate15 | 2 | 2TroutRedoPool14 | 10and14 | ||||||||||||
WRosenthal_Trout_plate22 | 4 | 2TroutRedoPool14 | 10and14 | ||||||||||||
WRosenthal_Trout_plate25 | 8 | 2TroutRedoPool15 | 15and16 | ||||||||||||
Wrosenthal_GY21_YCT_tray4 | 6 | 2TroutRedoPool15 | 15and16 | ||||||||||||
Wrosenthal_GY21_YCT_tray7tray1 | 7 | 82TroutRedoPool16 | 2TroutRedoPool1215and16 | ||||||||||||
Label reaction plates with MID plate used.
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