...
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 850500 | 25501500 |
0.45 | 10M dNTPs | 850500 | 382225 |
0.3 | Kapa HiFi HotStart DNA Pol | 850500 | 255150 |
7.25 | HPLC H2O | 850500 | 61633625 |
11 | Total Volume | 850500 | 93505500 |
Run on Thermocycler Program GSAF36 or AMF35:
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