We will be comparing the difference in results for 4AH1 using the 2 step PCR and the 1 step PCR. The 1 step PCR protocol used for 4AH1 is under the page named “NovaSeq4 1Step PCR and qPCR”. Below is the protocol used for the 2 step PCR. We will also be repeating the 2 step for PCR for GC2017-2.
*DO NOT USE THE FOLLOWING 2 STEP PCR MID PLATES (16S OR ITS) FOR 4AH1: A2, B2, A4, B4, C8, D8
4AH1 PCR1 MasterMix (make for 4 plates in 5mL tube)
...
Column 3 of GC2017-2_16S and GC2017-2_ITS was used for quantification.
GC2017-2_16S: 25.61
GC2017-2_ITS: 26.54
The full result report can be viewed below:
| View file | ||
|---|---|---|
|