Page Comparison

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• 515F (5’-GTGYCAGCMGCCGC GGTAA-3’) (Parada et al. 2016)

•  806R (5’-GGACTACNVGGGTWTCTAAT-3’) (Apprill et al. 2015)

• Together amplify a 292 bp region in E. coli

ITS Primer Base

• ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3')(Gardes et al 1993)

• ITS2 (3'-CGTAGCTACTTCTTGCGTCG-5') (White et al 1990)

• Together amplify a 256 bp region in Candida albacans

Primer structure

• 5'-FULL_ILLUMINA_FLOWCELL_SEQbarcodeGTGYCAGCMGCCGCGGTAA-3'

• 5'-FULL_ILLUMINA_FLOWCELL_SEQbarcodeGGACTACHVGGGTWTCTAAT-3'

• 5'-FULL_ILLUMINA_FLOWCELL_SEQbarcodeCTTGGTCATTTAGAGGAAGTAA-3'

• 5'-FULL_ILLUMINA_FLOWCELL_SEQbarcodeGCTGCGTTCTTCATCGATGC-3'

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