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Utilized this plate map and the parse report from above as input for this r-script and generated this report. The controls are Zymo Mock Community which is all bacterial and fungal DNA. “read percent” is the percent of reads a sample represented in the total number of reads produced. “read percent KJ only” is the same calculation limited to your samples.
samplename | wellposition | pair | forwardmid | reversemid | count | locus | readpercent | readpercentKJonly |
T10 | H1 | TTGGTAAGAA_CATACTAA | TTGGTAAGAA | CATACTAA | 389 | 16S | 0.0231711196118212 | 0.292241696654621 |
T10_B | H3 | CCGCGCGAA_TGCGAAGCAA | CCGCGCGAA | TGCGAAGCAA | 1437 | 16S | 0.0855961410853138 | 1.07956637041823 |
T12 | A2 | TCCTCTTGAA_CCAGAACCAA | TCCTCTTGAA | CCAGAACCAA | 2463 | 16S | 0.146710713634745 | 1.85036323614481 |
T12_B | E2 | GATGCGCA_TCGGAGCAA | GATGCGCA | TCGGAGCAA | 13188 | 16S | 0.785554564114905 | 9.90766965419318 |
T13 | B2 | ACGAGTCAA_AGACGCAA | ACGAGTCAA | AGACGCAA | 4787 | 16S | 0.285141772703825 | 3.59630077605571 |
T13_B | F2 | CTTCATCA_CCAACGCAA | CTTCATCA | CCAACGCAA | 5524 | 16S | 0.329041811659898 | 4.14998234529596 |
T14 | C2 | GGTAATGCAA_TAGATCAA | GGTAATGCAA | TAGATCAA | 1443 | 16S | 0.0859535362464216 | 1.08407395442833 |
T14_B | G2 | TCCGCTCA_ATCGCGCAA | TCCGCTCA | ATCGCGCAA | 1709 | 16S | 0.101798055055533 | 1.28391017887596 |
T15 | D2 | TCCATATCAA_AATAAGCAA | TCCATATCAA | AATAAGCAA | 16283 | 16S | 0.969910901386336 | 12.2328317394015 |
T15_B | H2 | GAGCTTCA_CCTTATCAA | GAGCTTCA | CCTTATCAA | 20964 | 16S | 1.24873869291059 | 15.7494985312789 |
T3 | A1 | TTATTCGAA_AACGCCAA | TTATTCGAA | AACGCCAA | 8083 | 16S | 0.481470847872367 | 6.07246692560233 |
T3_B | A3 | GGAATGGAA_CGGTCCAA | GGAATGGAA | CGGTCCAA | 0 | 16S | 0 | 0 |
T4 | B1 | CTTGCAGCAA_TGAAGCCAA | CTTGCAGCAA | TGAAGCCAA | 1960 | 16S | 0.116749085961875 | 1.47247744329835 |
T4_B | B3 | AGAACCGCAA_AACCGCCAA | AGAACCGCAA | AACCGCCAA | 2023 | 16S | 0.120501735153507 | 1.51980707540437 |
T5 | C1 | AATTACCA_ATCGGACCAA | AATTACCA | ATCGGACCAA | 2367 | 16S | 0.14099239105702 | 1.77824189198326 |
T5_B | C3 | ATAATCCA_CTGATCCAA | ATAATCCA | CTGATCCAA | 1370 | 16S | 0.0816052284529436 | 1.02923168230548 |
T6 | D1 | AGGCCAGAA_CTGATACCAA | AGGCCAGAA | CTGATACCAA | 592 | 16S | 0.0352629892293012 | 0.444748288996236 |
T6_B | D3 | CGAACGCA_ACCAAGCCAA | CGAACGCA | ACCAAGCCAA | 1373 | 16S | 0.0817839260334975 | 1.03148547431053 |
T7 | E1 | GCATCAGAA_TGAGAGAA | GCATCAGAA | TGAGAGAA | 8171 | 16S | 0.486712643568615 | 6.13857815775042 |
T7_B | E3 | ATAAGAGAA_TAGGAGCCAA | ATAAGAGAA | TAGGAGCCAA | 6801 | 16S | 0.405107415115671 | 5.10934647544494 |
T8 | F1 | CCGTCAAGAA_GGTAGGAA | CCGTCAAGAA | GGTAGGAA | 11775 | 16S | 0.701388003674022 | 8.84613361981534 |
T8_B | F3 | TCCATAGAA_GATACGCCAA | TCCATAGAA | GATACGCCAA | 7931 | 16S | 0.472416837124303 | 5.95827479734654 |
T9 | G1 | CTCCGAAGAA_GGCCATAA | CTCCGAAGAA | GGCCATAA | 12398 | 16S | 0.738497534569047 | 9.31417109286374 |
T9_B | G3 | ACTGACGAA_TTAACTCCAA | ACTGACGAA | TTAACTCCAA | 10431 | 16S | 0.621331487585879 | 7.83643480155361 |
T10 | H1 | TTATGCATCA_CTATCGGCCA | TTATGCATCA | CTATCGGCCA | 2408 | ITS | 0.146972925365464 | 1.79558114043264 |
T10_B | H3 | TAGACTCCA_GCTGCGTAA | TAGACTCCA | GCTGCGTAA | 1118 | ITS | 0.0682374296339654 | 0.833662672343726 |
T12 | A2 | GCTTGCCTCA_AAGTTGCTAA | GCTTGCCTCA | AAGTTGCTAA | 2641 | ITS | 0.161194142811541 | 1.96932300327351 |
T12_B | E2 | ACCTGAAC_CTGATGGA | ACCTGAAC | CTGATGGA | 11154 | ITS | 0.680787379371422 | 8.31723921942926 |
T13 | B2 | CGAGGAAGCA_TGCAACTTAA | CGAGGAAGCA | TGCAACTTAA | 6270 | ITS | 0.382691130415888 | 4.67537115885077 |
T13_B | F2 | GCGGTAAC_ACTGAATA | GCGGTAAC | ACTGAATA | 3661 | ITS | 0.223450116180633 | 2.72990969897172 |
T14 | C2 | CATCTCAGCA_CTTGCCGA | CATCTCAGCA | CTTGCCGA | 6794 | ITS | 0.414673610852559 | 5.06610393193495 |
T14_B | G2 | GACGAGAC_GTAGCATA | GACGAGAC | GTAGCATA | 2687 | ITS | 0.164001765139951 | 2.00362397190303 |
T15 | D2 | CAGGAACGCA_ACGTTCGA | CAGGAACGCA | ACGTTCGA | 17234 | ITS | 1.05188180886562 | 12.8509324643755 |
T15_B | H2 | TATTGGAC_GCCTTATA | TATTGGAC | GCCTTATA | 16781 | ITS | 1.02423283245758 | 12.5131424906977 |
T3 | A1 | GATCGTCCA_ATTCTGGAA | GATCGTCCA | ATTCTGGAA | 1558 | ITS | 0.0950929475578874 | 1.16175889401746 |
T3_B | A3 | TAGGCTAC_AATCCAGA | TAGGCTAC | AATCCAGA | 24595 | ITS | 1.50116241667923 | 18.3398331183309 |
T4 | B1 | TAACGGTAA_CTGGATGAA | TAACGGTAA | CTGGATGAA | 185 | ITS | 0.011291524581649 | 0.137949547749185 |
T4_B | B3 | ATATAGTA_GGAATAGA | ATATAGTA | GGAATAGA | 2999 | ITS | 0.183044768758732 | 2.23627401999896 |
T5 | C1 | TTCAATTA_CTTCAATAA | TTCAATTA | CTTCAATAA | 264 | ITS | 0.0161133107543532 | 0.196857733004243 |
T5_B | C3 | CCGCGTTA_AAGGAATAA | CCGCGTTA | AAGGAATAA | 6245 | ITS | 0.381165248715665 | 4.65672932807385 |
T6 | D1 | GTCAGACCA_ATAAGAACCA | GTCAGACCA | ATAAGAACCA | 3337 | ITS | 0.203674689345745 | 2.48831157210287 |
T6_B | D3 | TTGCGAAC_GATAGATAA | TTGCGAAC | GATAGATAA | 3650 | ITS | 0.222778728232535 | 2.72170729342987 |
T7 | E1 | AATGCAGGCA_GTCCTAACCA | AATGCAGGCA | GTCCTAACCA | 6262 | ITS | 0.382202848271817 | 4.66940577300215 |
T7_B | E3 | CTTGAGCCA_GCGCTATAA | CTTGAGCCA | GCGCTATAA | 4891 | ITS | 0.298523495831596 | 3.64708777319603 |
T8 | F1 | TTAATTGGCA_CTTATTACCA | TTAATTGGCA | CTTATTACCA | 19837 | ITS | 1.21075661149282 | 14.7919198848681 |
T8_B | F3 | GGAGCGCCA_GTAACCTAA | GGAGCGCCA | GTAACCTAA | 14275 | ITS | 0.871278450827242 | 10.6444853736196 |
T9 | G1 | AGAACTTGCA_CAGAGAGCCA | AGAACTTGCA | CAGAGAGCCA | 3568 | ITS | 0.217773836255804 | 2.66056208848159 |
T9_B | G3 | ATAATGCCA_ACCTAGTAA | ATAATGCCA | ACCTAGTAA | 7010 | ITS | 0.427857228742484 | 5.22716934984751 |
All samples amplified. There was one reaction failure, but the duplicate worked.