| Info |
|---|
02-04-2022 Illumina agreed there was likely a consumables problem. They are sending a replacement cartridge next week. |
...
%Q30 Read 1 | 78.8 |
%Q30 Read 2 | 75.2 |
%Clusters PF | 39 |
%Occupancy | 90.2 |
%Clusters Passing Filter is concerning. We will reach out to Illumina.
...
100/Results = ul of Pool to Add
100/10 4.98 = 10uL 20uL of Pool to Add
100 - uL of Pool to Add = ul of “10 mM Tris 8.5” to Add
100- 10 20 = 90uL 80uL of 10mM Tris 8.5
Dilute 1 nM full pool to loading concentration of 50 pM:
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