...
Reagent | ul/rxn | rxns | ul needed (x1.4) |
|---|---|---|---|
H2O | 9.52 | 272136 | 28561704 |
5x iProof buffer | 4 | 272136 | 1200716 |
10 mM dNTPs | 0.4 | 272136120 | 71.6 |
50 mM MgCl2 | 0.4 | 272136120 | 71.6 |
5 uM Illumina Primers | 1.33 | 272136 | 399238 |
iProof TAQ | 0.2 | 27213660 | 35.8 |
DMSO | 0.15 | 27213645 | 26.9 |
total | 16 | 272136 | 48002864 |
Add 16 ul of PCR1 MM to each well of four new hard shell PCR plates with 8 channel
...