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Add 1 ul of EcoR1 Adaptors with 96-channel and Track which template plate gets which MID plate.
CHP1: Eco5
CHP2:Eco6
PCR1:
Reagent | ul/rxn | rxns | ul needed (x1.4) |
|---|---|---|---|
H2O | 9.52 | 110 | 1047.2 |
5x iProof buffer | 4 | 110 | 440 |
10 mM dNTPs | 0.4 | 110 | 44 |
50 mM MgCl2 | 0.4 | 110 | 44 |
5 uM Illumina Primers | 1.33 | 110 | 146.3 |
iProof TAQ | 0.2 | 110 | 22 |
DMSO | 0.15 | 110 | 16.5 |
total | 16 | 110 | 1760 |
Add 16 ul of PCR1 MM to each well 80 wells of four a new hard shell PCR plates plate with 8 channel
Add 4uL of template from pooled plates with Benchsmart
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