Setup Notes
...
Normalize plate reader with Buffer AE for all plates
Quantify all plates
Check for samples above 150 ng/ul
Transfer 20 ul from all wells of a plate needing normalization to new VWR plate
Add 20 ul TE to all wells above 150 ng/ul
Restriction Digestion
(Keep MM and reaction plates on ice)
...
dilute 1 nM PhiX to 500 pM
...
Library
...
qPCR Result (nM)
...
RSB ul for 2nM
...
Library ul for 2 nM
...
RSB ul for load
...
Library ul for Load
...
PhiX ul for load
...
Loading Concentration Used
...
% Occupied
...
Samples
...
Yield Gbp
...
1
...
6.5
...
34.6
...
15.4
...
10.4
...
8
...
1.6
...
384
...
2
...
4.7
...
28.7
...
21.3
...
10.4
...
8
...
1.6
...
383
...
3
...
4.9
...
29.6
...
20.4
...
10.4
...
8
...
1.6
...
348
...
4
...
5.3
...
31.1
...
18.9
...
10.4
...
8
...
1.6
...
382
...
5
...
6
...
33.3
...
16.7
...
10.4
...
8
...
1.6
...
374
...
6
...
5.5
...
31.8
...
18.2
...
10.4
...
8
...
1.6
...
365
...
7
...
3.9
...
24.4
...
25.6
...
11.4
...
7
...
1.6
...
700pM
...
336
...
8
...
7.6
...
36.8
...
13.2
...
10.4
...
8
...
1.6
...
800 pM
...
98.97
...
297
...
126.52
...
1SMIN
...
900
...
99.23
...