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cutadapt -g ^GTGYCAGCMGCCGCGGTAA -o R1out_no16s YOURFORWARDSEQUENCES -e 0.25

cutadapt -g ^CTTGGTCATTTAGAGGAAGTAA ^CTTGGTCATTTAGAGGAAGTAAT -o R1out_no16s_noits R1out_no16s -e 0.25

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