Vortex and quick spin deep well plates.
Use the 50 uL Integra expandable spacing pipette to transfer 30 uL from each well into a transparent PCR plate. Label the plates as shown in this document Initial Sample Processing - Alfalfa 16S/ITS .
Coligos added during extraction so do NOT add during sample processing.
Seal, vortex, and spin aliquot plate.
Make a new folder under DNA Quantification labeled “Alfalfa Buerkle Lab 16S/ITS”. Check concentration on Synergy HTX and save file on petalibrary as plate name. Add a circle around the asterisk when concentrations have been measured. Normalize plates on the Hamilton Nimbus to 10ng/uL.
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