Pooling Calculations for NovaSeq4

Owned by Shannon Harris
Jan 28, 2021
1 min read

Below are all the plates that were pooled for NovaSeq4 along with their pooling quantities.

Plate

qPCR Result

Dilution 

Pool Amount

4AH1_16S

11.86

 

2uL

4AH1_ITS

15.74

 

2uL

4AH2_16S

36.27

 

2uL

4AH2_ITS

43.5

 

2uL

4PA1_16S

4.72

 

20uL

4PA1_ITS

22.76

 

2uL

4PA2_16S

8.58

 

20uL

4PA2_ITS

84.66

 

2uL

4PA3_16S

4.04

 

20uL

4PA3_ITS

46.07

 

2uL

4PA4_16S

71

 

2uL

4PA4_ITS

68.5

 

2uL

4PA5_16S

10.26

 

2uL

4PA5_ITS

41.22

 

2uL

4PA6_16S

11.57

 

2uL

4PA6_ITS

44.52

 

2uL

4MR1_16S

92.23

 

2uL

4MR1_ITS

53.47

 

2uL

4MR2_16S

105.34

40uL TE

2uL

4MR2_ITS

66.71

 

2uL

4SC1_16S

46.64

 

2uL

4SC1_ITS

47.77

 

2uL

4SC2_16S

65.97

 

2uL

4SC2_ITS

55.69

 

2uL

4LVD1_16S

112.54

40uL TE

2uL

4LVD1_ITS

24.71

 

2uL

4LVD2_16S

95.57

 

2uL

4LVD2_ITS

34.28

 

2uL

4LVD3_16S

112.47

40uL TE

2uL

4LVD3_ITS

101.03

40uL TE

2uL

4FB1_16S

28.57

 

2uL

4FB1_ITS

45.42

 

2uL

4FB2_16S

70.82

 

2uL

4FB2_ITS

71.52

 

2uL

4FB3_16S

73.04

 

2uL

4FB3_ITS

72.32

 

2uL

4AH12S_16S

3.71

 

20uL

4AH12S_ITS

13.58

 

2uL

GC2017-2_16S

25.61

 

2uL

GC2017-2_ITS

26.54

 

2uL

IC_GC5_16S*

31.33

 

1.33uL

IC_GC5_ITS*

18

 

1.33uL

AH3_ITS*

40.13

 

1.33uL

IC_GC1_16S*

25.29

 

1.33uL

*Plates missed during original pooling. Re-pooling occurred and amount added was adjusted to accommodate previous removal of pooled products.