Pooling Calculations for NovaSeq4
- Shannon Harris
Below are all the plates that were pooled for NovaSeq4 along with their pooling quantities.
Plate | qPCR Result | Dilution | Pool Amount |
4AH1_16S | 11.86 |
| 2uL |
4AH1_ITS | 15.74 |
| 2uL |
4AH2_16S | 36.27 |
| 2uL |
4AH2_ITS | 43.5 |
| 2uL |
4PA1_16S | 4.72 |
| 20uL |
4PA1_ITS | 22.76 |
| 2uL |
4PA2_16S | 8.58 |
| 20uL |
4PA2_ITS | 84.66 |
| 2uL |
4PA3_16S | 4.04 |
| 20uL |
4PA3_ITS | 46.07 |
| 2uL |
4PA4_16S | 71 |
| 2uL |
4PA4_ITS | 68.5 |
| 2uL |
4PA5_16S | 10.26 |
| 2uL |
4PA5_ITS | 41.22 |
| 2uL |
4PA6_16S | 11.57 |
| 2uL |
4PA6_ITS | 44.52 |
| 2uL |
4MR1_16S | 92.23 |
| 2uL |
4MR1_ITS | 53.47 |
| 2uL |
4MR2_16S | 105.34 | 40uL TE | 2uL |
4MR2_ITS | 66.71 |
| 2uL |
4SC1_16S | 46.64 |
| 2uL |
4SC1_ITS | 47.77 |
| 2uL |
4SC2_16S | 65.97 |
| 2uL |
4SC2_ITS | 55.69 |
| 2uL |
4LVD1_16S | 112.54 | 40uL TE | 2uL |
4LVD1_ITS | 24.71 |
| 2uL |
4LVD2_16S | 95.57 |
| 2uL |
4LVD2_ITS | 34.28 |
| 2uL |
4LVD3_16S | 112.47 | 40uL TE | 2uL |
4LVD3_ITS | 101.03 | 40uL TE | 2uL |
4FB1_16S | 28.57 |
| 2uL |
4FB1_ITS | 45.42 |
| 2uL |
4FB2_16S | 70.82 |
| 2uL |
4FB2_ITS | 71.52 |
| 2uL |
4FB3_16S | 73.04 |
| 2uL |
4FB3_ITS | 72.32 |
| 2uL |
4AH12S_16S | 3.71 |
| 20uL |
4AH12S_ITS | 13.58 |
| 2uL |
GC2017-2_16S | 25.61 |
| 2uL |
GC2017-2_ITS | 26.54 |
| 2uL |
IC_GC5_16S* | 31.33 |
| 1.33uL |
IC_GC5_ITS* | 18 |
| 1.33uL |
AH3_ITS* | 40.13 |
| 1.33uL |
IC_GC1_16S* | 25.29 |
| 1.33uL |
*Plates missed during original pooling. Re-pooling occurred and amount added was adjusted to accommodate previous removal of pooled products.