Trout-Pool3.2 Bioinformatics
- Gregg Randolph
Owned by Gregg Randolph
Feb 01, 2022
1 min read
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Demultiplexing
Split into 100000 line files
mkdir /gscratch/grandol1/trout1_2
cd /gscratch/grandol1/trout1_2
cat /project/microbiome/data_queue/seq/trout1_2/rawreads/TroutPool3-2_S1_L001_R1_001.fastq | split -l 1000000 -d --suffix-length=3 --additional-suffix=.fastq - Trout1_Pool3-2_
Parse split files
/project/microbiome/data_queue/seq/trout1_2/demultiplex/run_parsebarcodes_onSplitInput.pl
Recombine by sample name and mid
cd /project/microbiome/data_queue/seq/trout1_2/demultiplex
./run_splitFastq_gbs.sh
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