Murine B-cells Illumina Prep 1

Messed up with B1, T1, NB1, and NT1 added 10x less Resus than needed. Will add the correct amount to try and recover

36 total samples to be processed in 6 batches, maybe 35?
- Batch1: 2 sample batch
- Batch2: 2 sample batch
- Batch3: 16 samples
- Batch4: 16 samples

Sample	Processing #	Batch	Index	Cell Count	Error	ul needed for 2500 c/ul	Viability %

Sample	Processing #	Batch	Index	Cell Count	Error	ul needed for 2500 c/ul	Viability %
B1	1	1	1	3.73X10⁶	149	1492	55.5
B2	2	1	1	1.46 X10⁶	58	584	60.7
B3	3	2	2	1.45 X10⁶	58	580	58.7
B4	4	2	3	2.27 X10⁶	90	908	69.2
B5	5	3	4	2.94 X10⁶	118	1176	66.9
T1	6	3	2	1.13 X10⁶	45	452	59.2
T2	7	3	5	4.26 X10⁵	17	170.4	61.9
T3	8	3	6	1.09 X10⁵	4.4	43.6	23.3
T4	9	3	7	2.79 X10⁵	11	111.6	34.2
T5	10	3	8	3.99X10⁵	16	159.6	66.4
TL1	11	3		4.04 X10⁵	16	161.6	60.7
TL2	12	3	6	1.26 X10⁶	50	504	61.2
TL3	13	3	7	1.54 X10⁶	62	616	57.1
TL4	14	3	8	1.24 X10⁶	50	496	73.5
KL1	15	3	4	6.12 X10⁵	24	244.8	70
KL2	16	3	5	2.38 X10⁶	95	952	79.2
KL3	17	3		1.36 X10⁶	14	144	71.9
KS1	18	3	1	1.90 X10⁷	760	7600	77.5
KS2	19	3	2	1.36 X10⁷	544	5440	78.9
KS3	20	3	3	9.72 X10⁶	389	3888	75.1
NB1	21	4	3	180000	72	720	48.5
NB2	22	4	1	1800000	72	720	48.5
NB3	23	4	2	1800000	72	720	48.5
NT1	24	4	4	180000	7.2	72	44.6
NT2	25	4	3	180000	7.2	72	44.6
NT3	26	4	4	180000	7.2	72	44.6
NKT1	27	4	8	1070000	42.8	428	67.6
NKT2	28	4	1	1070000	42.8	428	67.6
NKT3	29	4	2	1070000	42.8	428	67.6
NL1	30	4	5	3390000	135.6	1356	48.5
NL2	31	4		3390000	135.6	1356	48.5
NL3	32	4		3390000	135.6	1356	48.5
NS1	33	4		6040000	241.6	2416	80.7
NS2	34	4		6040000	241.6	2416	80.7
NS3	35	4		6040000	241.6	2416	80.7
	36	4

1Cell Preparation

B1

B2

B3

B4

Thaw and stage capture and lysis materials

Thaw one PIP tube per sample and RNase Inhibitor on ice

Stage at RT Partitioning Reagent, Chemical Lysis Buffer 3

Stage Combination 1.5 ml/0.5 ml tube stand, P200 and P1000 wide and standard

Stage one 0.5 ml tube per sample

Reagent

Reagent