qPCR Fish vs Bac

Make 1 for each loci

Component	Stock Conc	Final Conc	V per	Reactions	V needed

Component	Stock Conc	Final Conc	V per	Reactions	V needed
2X MM	2X	1X	10	40	400
Primer1	10 uM	400 nM	0.8	40	32
Primer2	10 uM	400 nM	0.8	40	32
H2O			7.2	40	256

or

Component	Stock Conc	Final Conc	V per	Reactions	V needed

Component	Stock Conc	Final Conc	V per	Reactions	V needed
2X MM	2X	1X	10	55	550
Primer1	10 uM	400 nM	0.8	55	44
Primer2	10 uM	400 nM	0.8	55	44
H2O			7.2	55	352

Add 2 ul 10 ng/ul DNA from each sample

Temp	Duration	Cycles