micro NovaSeq Run #4

Pool was mailed to the University of Colorado Genomics and MicroArray Core at University of Colorado on 01-27-2021 promising sequencing to be run on Monday, Feb. 1st Monday, Feb. 8th. Feb. 22nd

QC from University of Colorado Genomics Core

R-script for Key. Demux Key.

Sequencing Report From University of Colorado Genomics Core:

Flowcell Summary

Clusters (Raw)	Clusters(PF)	Yield (MBases)

Clusters (Raw)	Clusters(PF)	Yield (MBases)
1,276,674,048	956,877,735	499,490

Lane Summary

Lane	PF Clusters	% of the lane	% Perfect barcode	% One mismatch barcode	Yield (Mbases)	% PF Clusters	% >= Q30 bases	Mean Quality Score

Lane	PF Clusters	% of the lane	% Perfect barcode	% One mismatch barcode	Yield (Mbases)	% PF Clusters	% >= Q30 bases	Mean Quality Score
1	486,226,470	100.00	100.00	NaN	253,810	76.17	89.20	35.01
2	470,651,265	100.00	100.00	NaN	245,680	73.73	87.99	34.77

Sample Onboarding

This will use the 1-step PCR primers. All of the adaptor sequence is added at once. This saves on time and reagents.

Include Yoon plate for resequencing