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(July 14th, 2021) The tubes to be plated from the most recent TN shipment, are currently arranged in 96 position tube racks awaiting processing. They are arranged per the “TubesToBePlated” sheet of this workbook. We are still missing the 10 samples listed below.

Alex Buerkle used the complicated TN spreadsheet to create a list of tubes that need to be amplicon sequenced. Shannon Harris had already arranged the tubes they had sent and transcribed the hand written labels into a spreadsheet and added them to 96 tube racks. I attempted to reconcile the tobeplated list from Alex’s data manipulation with this using this r-script and these data. I was able to locate 293 of the 303 tobeplated tubes and created a pull list. I found one transcription error. There are still 10 tubes I could not locate:

Site_101_Soil_3

Site_102_Soil_29

Site_102_Alf_Epi_7

Site_102_Trans_Epi_7

Site_111_Alf_Epi_10

Site_111_Trans_Epi_1

Site_127_Trans_Epi_9

Site_129_Alf_Epi_5

Site_129_Alf_Epi_25

Site_130_Alf_Epi_30

I tried to delve into the data to see if I could locate these 10 samples. I did not have any luck. I do not think it is a transcription error when I anti joined samplesInSites against our spreadsheet and then the spreadsheet against samplesInSites there was no easily discernible pattern. The list of sites between the 2 even disagreed. And, there were 212-228 disagreements.

Sites with Tube Samples Not Matching from TN spreadsheet

Sites with Tube Samples Not Matching from our spreadsheet

101

101

102

103

104

107

108

109

110

111

116

127

128

129

So, something is still fishy with the TN spreadsheet concerning tubes.

Samples in transcribed list but not TN list:

GTL Plate Name

modmodsamplename

well

ALF_PLT21_TN

Site_110_Trans_Endo_6

G3

ALF_PLT21_TN

Site_109_Trans_Endo_1

H3

ALF_PLT21_TN

Site_109_Trans_Endo_3

B4

ALF_PLT21_TN

Site_110_Trans_Endo_10

D4

ALF_PLT21_TN

Site_103_Trans_Endo_1

F4

ALF_PLT21_TN

Site_103_Trans_Endo_6

H4

ALF_PLT21_TN

Site_125_Trans_Endo_4

A5

ALF_PLT21_TN

Site_109_Soil_21

B5

ALF_PLT21_TN

Site_108_Soil_20

D5

ALF_PLT21_TN

Site_125_Trans_Endo_3

E5

ALF_PLT21_TN

Site_103_Trans_Endo_4

F5

ALF_PLT21_TN

Site_103_Trans_Endo_2

H5

ALF_PLT21_TN

Site_104_Trans_Endo_6

A6

ALF_PLT21_TN

Site_134_Trans_Endo_9

D6

ALF_PLT21_TN

Site_110_Trans_Endo_9

E6

ALF_PLT21_TN

Site_109_Soil_23

H6

ALF_PLT21_TN

Site_116_Trans_Endo_8

B7

ALF_PLT21_TN

Site_120_Soil_29

D7

ALF_PLT21_TN

Site_116_Trans_Endo_7

E7

ALF_PLT21_TN

Site_134_Trans_Endo_8

G7

ALF_PLT21_TN

Site_104_Trans_Endo_1

H7

ALF_PLT21_TN

Site_101_Soil_13

A8

ALF_PLT21_TN

Site_126_Trans_Endo_7

D8

ALF_PLT21_TN

Site_103_Trans_Endo_10

A9

ALF_PLT21_TN

Site_104_Trans_Endo_9

C9

ALF_PLT21_TN

Site_125_Trans_Endo_9

F9

ALF_PLT21_TN

Site_109_Soil_12

G9

ALF_PLT21_TN

Site_104_Trans_Endo_5

H9

ALF_PLT21_TN

Site_110_Soil_3

C10

ALF_PLT21_TN

Site_110_Trans_Endo_7

D10

ALF_PLT21_TN

Site_116_Trans_Endo_10

E10

ALF_PLT21_TN

Site_110_Trans_Endo_5

G10

ALF_PLT21_TN

Site_103_Trans_Endo_8

A11

ALF_PLT21_TN

Site_110_Soil_4

E11

ALF_PLT21_TN

Site_103_Trans_Endo_5

G11

ALF_PLT21_TN

Site_109_Trans_Endo_7

B12

ALF_PLT22_TN

Site_105_Alf_Epi_4

B1

ALF_PLT22_TN

Site_106_Alf_Epi_4

C1

ALF_PLT22_TN

Site_105_Alf_Epi_12

A2

ALF_PLT22_TN

Site_106_Alf_Epi_24

F2

ALF_PLT22_TN

Site_106_Trans_Epi_9

H2

ALF_PLT22_TN

Site_105_Alf_Epi_24

A3

ALF_PLT22_TN

Site_105_Alf_Epi_26

G3

ALF_PLT22_TN

Site_107_Trans_Epi_9

D4

ALF_PLT22_TN

Site_106_Alf_Epi_29

F4

ALF_PLT22_TN

Site_107_Alf_Epi_2

D5

ALF_PLT22_TN

Site_107_Alf_Epi_30

E5

ALF_PLT22_TN

Site_106_Alf_Epi_21

G5

ALF_PLT22_TN

Site_107_Alf_Epi_17

A6

ALF_PLT22_TN

Site_106_Alf_Epi_22

B6

ALF_PLT22_TN

Site_107_Alf_Epi_3

C6

ALF_PLT22_TN

Site_107_Alf_Epi_29

D6

ALF_PLT22_TN

Site_107_Alf_Epi_6

F6

ALF_PLT22_TN

Site_106_Trans_Epi_1

H6

ALF_PLT22_TN

Site_105_Alf_Epi_10

A7

ALF_PLT22_TN

Site_107_Trans_Epi_1

E7

ALF_PLT22_TN

Site_107_Alf_Epi_25

G7

ALF_PLT22_TN

Site_107_Alf_Epi_4

F8

ALF_PLT22_TN

Site_107_Alf_Epi_24

A9

ALF_PLT22_TN

Site_107_Alf_Epi_10

C9

ALF_PLT22_TN

Site_105_Alf_Epi_20

D9

ALF_PLT22_TN

Site_105_Alf_Epi_18

E9

ALF_PLT22_TN

Site_107_Alf_Epi_12

H9

ALF_PLT22_TN

Site_105_Alf_Epi_1

C10

ALF_PLT22_TN

Site_105_Alf_Epi_14

G11

ALF_PLT22_TN

Site_106_Trans_Epi_7

A12

ALF_PLT22_TN

Site_105_Alf_Epi_15

C12

ALF_PLT22_TN

Site_106_Alf_Epi_5

D12

ALF_PLT23_TN

Site_108_Soil_1

B1

ALF_PLT23_TN

Site_104_Trans_Endo_4

F1

ALF_PLT23_TN

Site_134_Trans_Endo_5

A2

ALF_PLT23_TN

Site_126_Trans_Endo_9

B2

ALF_PLT23_TN

Site_109_Trans_Endo_2

C2

ALF_PLT23_TN

Site_108_Soil_17

D2

ALF_PLT23_TN

Site_116_Trans_Endo_3

F2

ALF_PLT23_TN

Site_134_Trans_Endo_3

G2

ALF_PLT23_TN

Site_116_Trans_Endo_9

H2

ALF_PLT23_TN

Site_126_Trans_Endo_6

A3

ALF_PLT23_TN

Site_126_Trans_Endo_1

C3

ALF_PLT23_TN

Site_126_Trans_Endo_5

D3

ALF_PLT23_TN

Site_109_Soil_7

E3

ALF_PLT23_TN

Site_126_Trans_Endo_10

G3

ALF_PLT23_TN

Site_134_Trans_Endo_4

H3

ALF_PLT23_TN

Site_134_Trans_Endo_6

A4

ALF_PLT23_TN

Site_104_Trans_Endo_10

B4

ALF_PLT23_TN

Site_110_Trans_Endo_3

D4

ALF_PLT23_TN

Site_116_Trans_Endo_6

E4

ALF_PLT23_TN

Site_125_Trans_Endo_7

G4

ALF_PLT23_TN

Site_134_Trans_Endo_1

H4

ALF_PLT23_TN

Site_126_Trans_Endo_4

E5

ALF_PLT23_TN

Site_109_Trans_Endo_4

F5

ALF_PLT23_TN

Site_103_Trans_Endo_3

G5

ALF_PLT23_TN

Site_126_Trans_Endo_2

H5

ALF_PLT23_TN

Site_116_Trans_Endo_5

C6

ALF_PLT23_TN

Site_110_Soil_27

D6

ALF_PLT23_TN

Site_109_Trans_Endo_8

G6

ALF_PLT23_TN

Site_134_Trans_Endo_2

D7

ALF_PLT23_TN

Site_110_Trans_Endo_1

E7

ALF_PLT23_TN

Site_109_Trans_Endo_9

F7

ALF_PLT23_TN

Site_110_Soil_13

A8

ALF_PLT23_TN

Site_103_Trans_Endo_7

C8

ALF_PLT23_TN

Site_125_Trans_Endo_6

E8

ALF_PLT23_TN

Site_108_Soil_8

G8

ALF_PLT23_TN

Site_134_Trans_Endo_7

H8

ALF_PLT23_TN

Site_104_Trans_Endo_7

C10

ALF_PLT23_TN

Site_125_Trans_Endo_10

F10

ALF_PLT23_TN

Site_103_Trans_Endo_9

H10

ALF_PLT23_TN

Site_110_Trans_Endo_2

A11

ALF_PLT23_TN

Site_109_Trans_Endo_10

E11

ALF_PLT23_TN

Site_116_Trans_Endo_4

F11

ALF_PLT23_TN

Site_109_Soil_22

G11

ALF_PLT23_TN

Site_104_Trans_Endo_3

E12

ALF_PLT23_TN

Site_104_Trans_Endo_8

G12

ALF_PLT23_TN

Site_107_Alf_Epi_21

H12

ALF_PLT24_TN

Site_105_Trans_Epi_2

A1

ALF_PLT24_TN

Site_105_Alf_Epi_9

C1

ALF_PLT24_TN

Site_129__Alf_Epi_25

G1

ALF_PLT24_TN

Site_105_Alf_Epi_11

F2

ALF_PLT24_TN

Site_107_Alf_Epi_13

H2

ALF_PLT24_TN

Site_106_Alf_Epi_23

A3

ALF_PLT24_TN

Site_105_Alf_Epi_2

B3

ALF_PLT24_TN

Site_107_Alf_Epi_26

C3

ALF_PLT24_TN

Site_105_Alf_Epi_28

H3

ALF_PLT24_TN

Site_106_Alf_Epi_18

D4

ALF_PLT24_TN

Site_106_Alf_Epi_10

G4

ALF_PLT24_TN

Site_106_Alf_Epi_11

D5

ALF_PLT24_TN

Site_105_Alf_Epi_27

B6

ALF_PLT24_TN

Site_106_Alf_Epi_17

G6

ALF_PLT24_TN

Site_105_Alf_Epi_13

B7

ALF_PLT24_TN

Site_106_Alf_Epi_28

F7

ALF_PLT24_TN

Site_106_Alf_Epi_3

G7

ALF_PLT24_TN

Site_105_Alf_Epi_7

D8

ALF_PLT24_TN

Site_105_Trans_Epi_7

G8

ALF_PLT24_TN

Site_107_Alf_Epi_8

C9

ALF_PLT24_TN

Site_106_Trans_Epi_6

F9

ALF_PLT24_TN

Site_107_Trans_Epi_3

G9

ALF_PLT24_TN

Site_105_Alf_Epi_6

E10

ALF_PLT24_TN

Site_107_Trans_Epi_10

F10

ALF_PLT24_TN

Site_106_Trans_Epi_3

G10

ALF_PLT24_TN

Site_105_Trans_Epi_9

C11

ALF_PLT24_TN

Site_107_Trans_Epi_2

G11

ALF_PLT24_TN

Site_107_Alf_Epi_18

H11

ALF_PLT24_TN

Site_106_Trans_Epi_2

A12

ALF_PLT24_TN

Site_107_Alf_Epi_19

E12

ALF_PLT24_TN

Site_107_Alf_Epi_28

H12

ALF_PLT25_TN

Site_107_Alf_Epi_16

B1

ALF_PLT25_TN

Site_105_Alf_Epi_30

C1

ALF_PLT25_TN

Site_106_Trans_Epi_8

D1

ALF_PLT25_TN

Site_106_Trans_Epi_10

E1

ALF_PLT25_TN

Site_106_Alf_Epi_9

B2

ALF_PLT25_TN

Site_106_Alf_Epi_7

C2

ALF_PLT25_TN

Site_107_Alf_Epi_7

F2

ALF_PLT25_TN

Site_105_Alf_Epi_3

H2

ALF_PLT25_TN

Site_107_Trans_Epi_7

B3

ALF_PLT25_TN

Site_120_Trans_Epi_7

H3

ALF_PLT25_TN

Site_105_Alf_Epi_23

A4

ALF_PLT25_TN

Site_107_Trans_Epi_8

B4

ALF_PLT25_TN

Site_105_Alf_Epi_29

F4

ALF_PLT25_TN

Site_105_Alf_Epi_21

G4

ALF_PLT25_TN

Site_106_Alf_Epi_20

H4

ALF_PLT25_TN

Site_105_Alf_Epi_16

B5

ALF_PLT25_TN

Site_107_Alf_Epi_11

F7

ALF_PLT25_TN

Site_107_Alf_Epi_1

G7

ALF_PLT25_TN

Site_105_Alf_Epi_5

H7

ALF_PLT25_TN

Site_107_Alf_Epi_9

G8

ALF_PLT25_TN

Site_106_Alf_Epi_8

H8

ALF_PLT25_TN

Site_107_Alf_Epi_23

A9

ALF_PLT25_TN

Site_107_Alf_Epi_22

D9

ALF_PLT25_TN

Site_106_Alf_Epi_13

G9

ALF_PLT25_TN

Site_106_Alf_Epi_16

B10

ALF_PLT25_TN

Site_105_Trans_Epi_10

C10

ALF_PLT25_TN

Site_106_Alf_Epi_30

D10

ALF_PLT25_TN

Site_105_Trans_Epi_4

B11

ALF_PLT25_TN

Site_106_Alf_Epi_15

F11

ALF_PLT25_TN

Site_105_Alf_Epi_8

G11

ALF_PLT25_TN

Site_107_Alf_Epi_14

A12

ALF_PLT25_TN

Site_105_Trans_Epi_3

B12

ALF_PLT25_TN

Site_107_Alf_Epi_20

G12

ALF_PLT25_TN

Site_129_Alf_Epi_5_

H12

ALF_PLT26_TN

Site_105_Alf_Epi_22

A1

Samples in TN list, but not transcribed one:

Site

samplename

container

modsamplename

modmodsamplename

102

17

Tube

Alf Endo 17

Site_102_Alf_Endo_17

102

18

Tube

Alf Endo 18

Site_102_Alf_Endo_18

102

19

Tube

Alf Endo 19

Site_102_Alf_Endo_19

102

20

Tube

Alf Endo 20

Site_102_Alf_Endo_20

102

21

Tube

Alf Endo 21

Site_102_Alf_Endo_21

102

22

Tube

Alf Endo 22

Site_102_Alf_Endo_22

102

23

Tube

Alf Endo 23

Site_102_Alf_Endo_23

102

24

Tube

Alf Endo 24

Site_102_Alf_Endo_24

102

25

Tube

Alf Endo 25

Site_102_Alf_Endo_25

102

26

Tube

Alf Endo 26

Site_102_Alf_Endo_26

102

27

Tube

Alf Endo 27

Site_102_Alf_Endo_27

102

28

Tube

Alf Endo 28

Site_102_Alf_Endo_28

102

29

Tube

Alf Endo 29

Site_102_Alf_Endo_29

102

30

Tube

Alf Endo 30

Site_102_Alf_Endo_30

102

Soil 29

Tube

Soil 29

Site_102_Soil_29

102

7 epiphyte

Tube

Alf Epi 7

Site_102_Alf_Epi_7

102

Trans  7 epiphyte

Tube

Trans Epi 7

Site_102_Trans_Epi_7

111

1

x

Alf Endo 1

Site_111_Alf_Endo_1

111

2

Tube

Alf Endo 2

Site_111_Alf_Endo_2

111

3

Tube

Alf Endo 3

Site_111_Alf_Endo_3

111

4

Tube

Alf Endo 4

Site_111_Alf_Endo_4

111

5

x

Alf Endo 5

Site_111_Alf_Endo_5

111

6

x

Alf Endo 6

Site_111_Alf_Endo_6

111

7

Tube

Alf Endo 7

Site_111_Alf_Endo_7

111

8

Tube

Alf Endo 8

Site_111_Alf_Endo_8

111

9

Tube

Alf Endo 9

Site_111_Alf_Endo_9

111

10

Tube

Alf Endo 10

Site_111_Alf_Endo_10

111

11

Tube

Alf Endo 11

Site_111_Alf_Endo_11

111

12

Tube

Alf Endo 12

Site_111_Alf_Endo_12

111

13

Tube

Alf Endo 13

Site_111_Alf_Endo_13

111

14

Tube

Alf Endo 14

Site_111_Alf_Endo_14

111

15

Tube

Alf Endo 15

Site_111_Alf_Endo_15

111

16

Tube

Alf Endo 16

Site_111_Alf_Endo_16

111

17

Tube

Alf Endo 17

Site_111_Alf_Endo_17

111

18

Tube

Alf Endo 18

Site_111_Alf_Endo_18

111

19

Tube

Alf Endo 19

Site_111_Alf_Endo_19

111

20

Tube

Alf Endo 20

Site_111_Alf_Endo_20

111

21

Tube

Alf Endo 21

Site_111_Alf_Endo_21

111

22

Tube

Alf Endo 22

Site_111_Alf_Endo_22

111

23

Tube

Alf Endo 23

Site_111_Alf_Endo_23

111

24

Tube

Alf Endo 24

Site_111_Alf_Endo_24

111

25

Tube

Alf Endo 25

Site_111_Alf_Endo_25

111

26

Tube

Alf Endo 26

Site_111_Alf_Endo_26

111

27

Tube

Alf Endo 27

Site_111_Alf_Endo_27

111

28

Tube

Alf Endo 28

Site_111_Alf_Endo_28

111

29

Tube

Alf Endo 29

Site_111_Alf_Endo_29

111

30

Tube

Alf Endo 30

Site_111_Alf_Endo_30

111

Soil

x

Soil

Site_111_Soil

111

1 epiphyte

x

Alf Epi 1

Site_111_Alf_Epi_1

111

5 epiphyte

x

Alf Epi 5

Site_111_Alf_Epi_5

111

10 epiphyte

Tube

Alf Epi 10

Site_111_Alf_Epi_10

111

Trans  1 epiphyte

Tube

Trans Epi 1

Site_111_Trans_Epi_1

127

1

Tube

Alf Endo 1

Site_127_Alf_Endo_1

127

2

Tube

Alf Endo 2

Site_127_Alf_Endo_2

127

3

Tube

Alf Endo 3

Site_127_Alf_Endo_3

127

4

X

Alf Endo 4

Site_127_Alf_Endo_4

127

5

Tube

Alf Endo 5

Site_127_Alf_Endo_5

127

6

Tube

Alf Endo 6

Site_127_Alf_Endo_6

127

7

Tube

Alf Endo 7

Site_127_Alf_Endo_7

127

8

X

Alf Endo 8

Site_127_Alf_Endo_8

127

9

Tube

Alf Endo 9

Site_127_Alf_Endo_9

127

10

Tube

Alf Endo 10

Site_127_Alf_Endo_10

127

11

Tube

Alf Endo 11

Site_127_Alf_Endo_11

127

12

Tube

Alf Endo 12

Site_127_Alf_Endo_12

127

13

Tube

Alf Endo 13

Site_127_Alf_Endo_13

127

14

Tube

Alf Endo 14

Site_127_Alf_Endo_14

127

15

Tube

Alf Endo 15

Site_127_Alf_Endo_15

127

16

Tube

Alf Endo 16

Site_127_Alf_Endo_16

127

17

Tube

Alf Endo 17

Site_127_Alf_Endo_17

127

18

Tube

Alf Endo 18

Site_127_Alf_Endo_18

127

19

Tube

Alf Endo 19

Site_127_Alf_Endo_19

127

20

Tube

Alf Endo 20

Site_127_Alf_Endo_20

127

21

Tube

Alf Endo 21

Site_127_Alf_Endo_21

127

22

Tube

Alf Endo 22

Site_127_Alf_Endo_22

127

23

Tube

Alf Endo 23

Site_127_Alf_Endo_23

127

24

Tube

Alf Endo 24

Site_127_Alf_Endo_24

127

25

Tube

Alf Endo 25

Site_127_Alf_Endo_25

127

26

Tube

Alf Endo 26

Site_127_Alf_Endo_26

127

27

Tube

Alf Endo 27

Site_127_Alf_Endo_27

127

28

Tube

Alf Endo 28

Site_127_Alf_Endo_28

127

29

Tube

Alf Endo 29

Site_127_Alf_Endo_29

127

30

Tube

Alf Endo 30

Site_127_Alf_Endo_30

127

4 epiphyte

X

Alf Epi 4

Site_127_Alf_Epi_4

127

8 epiphyte

X

Alf Epi 8

Site_127_Alf_Epi_8

127

Trans  9 epiphyte

Tube

Trans Epi 9

Site_127_Trans_Epi_9

128

1

Tube

Alf Endo 1

Site_128_Alf_Endo_1

128

3

Tube

Alf Endo 3

Site_128_Alf_Endo_3

128

4

Tube

Alf Endo 4

Site_128_Alf_Endo_4

128

5

Tube

Alf Endo 5

Site_128_Alf_Endo_5

128

6

Tube

Alf Endo 6

Site_128_Alf_Endo_6

128

7

Tube

Alf Endo 7

Site_128_Alf_Endo_7

128

8

Tube

Alf Endo 8

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