Reagents and Ordering
EcoR1 (20,000 units/ml)
MseI (10,000 units/ml)
T4 DNA ligase buffer (400,000 units/ml)
DNA polymerase (BioRad iProof or KAPA HiFi)
BSA (1 mg/ml)-We have this in 20 mg/ul
1 M NaCl-We have this at 5M
DMSO
EcoR1 adaptors (We need 1 uM MIDed in plates)
Mse1 Adaptors (10 uM stock probably need to dilute from 100 uM)
IIllpcr2 (2.5 uM working)
Illpcr1 (2.5 uM working)