Pool Makeup Info
Pool 2 has all 8 MID plates in it. It is therefore the best confirmatory test.
Dilution and Prep for iSeq Sequencing
The aliquot of Pool2 size selected for the 300-450 bp range without dilution of initial pool qPCRs a concentration of 10.6 nM.
Dilute non-diluted pool to 1 nM: Add 9.5 ul Pool 2 to 90.5 ul TE.
Dilute to loading concentration: Add 10 ul from above to 90 ul 10uM Tris
Equilibrate FlowCell to RT ~10 minutes
Load 20ul onto iSeq cartridge
Start Run