Paper detailing target and base primers
Fwd Primer Base (WANDA): CAGCCGCGGTAATTCCAGCT*
Rev Primer Base (AML2): GAACCCAAACACTTTGGTTTCC
200 sample multiplex required. Sticking to full columns for ease of handling, we will need 16 forward and 16 reverse primers for 256 possible combinations.
Design and sequences of 2-step sequencing primers.
Test Samples: rhizosphere extractions