Reagents and Ordering
- EcoR1 (20,000 units/ml)
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- MseI (10,000 units/ml)
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- T4 DNA ligase buffer (400,000 units/ml)
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- DNA polymerase (BioRad iProof or KAPA HiFi)
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- BSA (1 mg/ml)-We have this in 20 mg/ul
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- 1 M NaCl-We have this at 5M
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- DMSO
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- EcoR1 adaptors (We need 1 uM MIDed in plates)
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- Mse1 Adaptors (10 uM stock probably need to dilute from 100 uM)
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- IIllpcr2 (2.5 uM working)
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- Illpcr1 (2.5 uM working)