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Reagents and Ordering

  • EcoR1 (20,000 units/ml)
  • MseI (10,000 units/ml)
  • T4 DNA ligase buffer (400,000 units/ml)
  • DNA polymerase (BioRad iProof or KAPA HiFi)
  • BSA (1 mg/ml)-We have this in 20 mg/ul
  • 1 M NaCl-We have this at 5M
  • DMSO
  • EcoR1 adaptors (We need 1 uM MIDed in plates)
  • Mse1 Adaptors (10 uM stock probably need to dilute from 100 uM)
  • IIllpcr2 (2.5 uM working)
  • Illpcr1 (2.5 uM working)

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