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Product | Quantity | Unit Cost | Cost for 200 | Cost for 96 | Cost for 24 | Reason |
|---|---|---|---|---|---|---|
TruSeq Nano DNA High Throughput Library Prep Kit (96 samples) | 1 | $2840 | $2840 | Base library prep | ||
Nextera XT DNA Library Preparation Kit (96 samples) | 2 | $3435 | $6870 | 0 | Base library prep | |
Nextera XT DNA Library Preparation Kit (24 samples) | 1 | $905 | 0 | $905 | Base library prep | |
IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) | 1 | $672 | $672 | $672 (same kit can be used for the 200 sample run) | multiplexing 1-96 samples | |
IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) | 1 | $672 | $672 | 0 | multiplexing +1-96 samples | |
TruSeq DNA CD Indexes (96 Indexes, 96 Samples) | 1 | $546 | $546 | multiplexing +1-96 samples | ||
iSeq 2x150 bp sequencing | 1 | $600 | $600 | $600 | $600 | sequencing |
qPCR standards | 1 | $12 | $12 | $12 | $12 | standard curve |
qPCR plate check | 2 | $0.05 | $0.10 | $0.05 | qc | |
bioanalyzer check | 1 | $40 | $40 | $40 | $40 | qc |
Reamplification steps | 192 | $10 | $1920 | $960 | $240 | |
Total Estimate | $10786 | $4998 | $2470 |
Questions:
Are John Calder’s kits available?
How many samples?200 sample multiplex level, smaller proof of conceptWhich Illumina Kit is best? (TruSeq or Nextera). Nextera allows for higher multiplexing, I believe.
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