...
Step | Temp C | Cycles | Time |
---|---|---|---|
Denature | 95 | 1X | 10:00 |
Annealing | 9495 | 50X | 0:30 |
Annealing | 55 | 50X | 0:30 |
Extension/Elongation | 72 | 50X | 1:00 |
Extension/Elongation | 7472 | 1X | 9:00 |
Hold | 4 | 1X | 0:00 |
MagBead Cleanup:
...
...
Step | Temp C | Cycles | Time |
---|---|---|---|
Denature | 95 | 1X | 10:00 |
Annealing | 9495 | 50X | 0:30 |
Annealing | 55 | 50X | 0:30 |
Extension/Elongation | 72 | 50X | 1:00 |
Extension/Elongation | 7472 | 1X | 9:00 |
Hold | 4 | 1X | 0:00 |
...