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Step

Temp C

Cycles

Time

Denature

95

1X

10:00

Annealing

9495

50X

0:30

Annealing

55

50X

0:30

Extension/Elongation

72

50X

1:00

Extension/Elongation

7472

1X

9:00

Hold

4

1X

0:00

MagBead Cleanup:

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