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ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 220390 | 6601170 |
0.45 | 10M dNTPs | 220390 | 99176 |
0.3 | Kapa HiFi HotStart DNA Pol | 220390 | 66117 |
7.25 | HPLC H2O | 220390 | 15952827 |
11 | Total Volume | 220390 | 24204290 |
Add 11 ul to each well of a hard shell, full skirt plate. Add 2 uL of primers and 2uL of template to each well.
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