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Run on thermocycler program AMFAMF35:

Step

Temp C

Cycles

Time

Denature

95

1X

10:00

Denature

95

35X

0:30

Annealing

55

35X

0:30

Extension/Elongation

72

35X

1:00

Extension/Elongation

72

1X

9:00

Hold

4

1X

0:00

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