...
Make 1:1000 dilutions of column 1,46,8,11 10 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 | BS_AMF_Pool |
| NTC | NTC | NTC | |||||||
B |
|
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 | BS_AMF_Pool |
|
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C |
|
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 | BS_AMF_Pool |
|
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | |
E |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | |
F |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 2 pM Std | 2 pM Std | 2 pM Std | |
G |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 20 pM Std | 20 pM Std | 20 pM Std | |
H |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
|
|
|
|
Add 16 ul of Illumina Library Quantification MasterMix to each well:
...
Add 4 ul of template, pool, or standards to each well:
...
...
1
...
2
...
3
...
4
...
5
...
6
...
8
9
10
11
12
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AS1 | BS_PLT1_ACol1S2 | BS_PLT1_ACol6S3 | BS_PLT1_ACol10S4 | BS_PLT2_ACol1S5 | BS_PLT2_ACol6S6 | BS_PLT2_ACol10S7 | BS_AS8AMF_APool |
| NTC | NTC | NTC | |||||||
B |
|
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 | BS_AMF_Pool |
|
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C |
|
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 | BS_AMF_Pool |
|
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | |
E |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | |
F |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 2 pM Std | 2 pM Std | 2 pM Std | |
G |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
| 20 pM Std | 20 pM Std | 20 pM Std | |
H |
|
|
|
|
| BS_PLT1_Col1 | BS_PLT1_Col6 | BS_PLT1_Col10 | BS_PLT2_Col1 | BS_PLT2_Col6 | BS_PLT2_Col10 |
|
|
|
|
|
|
Results:
| View file | ||
|---|---|---|
|
...