...
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | S1_A | S2_A | S3_A | S4_A | S5_A | S6_A | S7_A | S8_A |
| NTC | NTC | NTC |
B |
|
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| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C |
|
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|
|
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D |
|
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|
|
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std |
E |
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| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std |
F |
|
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| 2 pM Std | 2 pM Std | 2 pM Std |
G |
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|
|
| 20 pM Std | 20 pM Std | 20 pM Std |
H |
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Results:
| View file | ||
|---|---|---|
|
There were some outliers, but most samples generated 10s of nano moles (~50).
The pool via standard size estimation returned a mean of 42.66 nM. This should be adjusted for the difference between the standards' fragment sizes and the expected product size (452 vs