...
Since the test sequencing included all samples, we are adjusting pooling for samples that both replicates returned less than 500 reads each. These samples will be pooled at a volume of 20 ul while all others will be pooled at 2 ul. 2 ul from all was combined into one column of strip tubes. 18 ul was added by well pattern above. Tubes were vortexed and centrifuged. 100 ul was added from each strip tube to a 1.5 ml tube which was vortexed and centrifuged before 40 ul was transferred to a tube for shipping.