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Pooling for CU sequencing:

Code Block
library(tidyverse)
AmfReads <- read.csv("/Users/gregg/Downloads/AMF1_Test_filtermergestats.csv", header = FALSE)
Map <- read.csv("/Users/gregg/Downloads/Bo_Stevens_Sample_Positions.csv", header=TRUE)
Map <- Map[,c(1,2,4)]
Map$order <- c(1:96, 1:92)
AMFmeta <- data.frame(do.call('rbind', strsplit(as.character(AmfReads$V1),'.',fixed=TRUE)))
AmfReads <- cbind(AMFmeta$X2, AmfReads[,2:4])
names(AmfReads) <- c("Sample.ID", "Reads", "Reads2", "Reads3")
AmfReads <- inner_join(AmfReads, Map, by = "Sample.ID")
LowAmf <- AmfReads[ which(AmfReads$Reads < 500),]
write.csv(LowAmf, "/Users/gregg/Downloads/LowAmf.csv", quote = FALSE, row.names = FALSE)

AMF1_Test_filtermergestats.csv Bo_Stevens_Sample_Positions.csv

Sample.ID

Reads

Reads2

Reads3

Plate.Position

Plate

order

A26

35

26

18

A9

2

65

A27

42

20

15

B9

2

66

A29

16

7

4

C9

2

68

A30

36

24

11

D9

2

69

A31

40

20

15

E9

2

70

A32

28

18

11

F9

2

71

A34

36

12

8

G9

2

73

A35

34

18

10

H9

2

74

B3

392

248

149

G11

2

91

L108

77

47

32

F4

1

30

L54

70

33

19

F7

1

54

L88

416

236

143

A4

1

25

LB1

104

51

34

A2

1

9

LB2

164

107

45

F3

1

22

LB3

70

35

20

C5

1

35

LB4

308

194

159

H6

1

48

LB5

310

169

129

E8

1

61

LB6

118

68

50

B10

1

74

LB7

374

219

161

G11

1

87

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