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34-2906-2022 We will be pooling All samples have been pooled and should be mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus pooling normalization protocol, Normalization_Tracking_Adaptive . We will be normalizing to 10 ng/ul. This will leave , leaving samples below this as is. We will normalize normalized in 20 ul, unless the starting concentration is was above 100 ng/ul. We will normalize then normalized in 50 ul for those samples only. 2 ul then. The Nimbus program will handle this volume switch on its ownfrom each sample was added to a new plate and then this plate was pooled into a column and the column was pooled into a 15 ml tube.