micro NovaSeq Run #5
- Gregg Randolph
- Shannon Harris
10-21-2022 Contamination has been detected in most blanks likely from an Alfalfa 16S/ITS project that bookended this one. Many plates will need to be rerun.
5-02-2022 Data have been processed and can be found in (/project/microbiome/data/seq/cu_29april22novaseq5) on Teton.
Data were returned to us 4-29-22. Report confusion has been cleared up. It involved using the index reads they ran in their calculations.
All samples have been pooled and were mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus normalization protocol, Normalization_Tracking_Adaptive to 10 ng/ul, leaving samples below this as is. We normalized in 20 ul, unless the starting concentration was above 100 ng/ul. We then normalized in 50 ul for those samples only. 2 ul from each sample was added to a new plate and then this plate was pooled into a column and the column was pooled into a 15 ml tube.
Flowcell Summary
Clusters (Raw) | Clusters(PF) | Yield (MBases) |
|---|---|---|
985,502,477 | 956,877,735 | 494,722 |
Lane Summary
Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|
1 | 492,110,025 | 100.00 | 100.00 | NaN | 247,039 | 77.09 | 87.48 | 34.69 |
2 | 493,392,452 | 100.00 | 100.00 | NaN | 247,683 | 77.29 | 87.47 | 34.69 |
Include Yoon plate for resequencing(16S0C8, 16S0D8, ITS0C8, ITS0D8). This just needs to be added to the pool.
In MISO | Researcher | GTL Plate Name | |||||||
|---|---|---|---|---|---|---|---|---|---|
X | Felix B. | 5FB1 | 5FB2 | 5FB3 | 5FB4 | 5FB5 |
|
|
|
X | Ayda L. | 5AL1 | 5AL2 | 5AL3 | 5AL4 | 5AL5 | 5AL6 | 5AL7 | 5AL8 |
X | Linda VD | 5LVD1 | 5LVD2 | 5LVD3 | 5LVD4 | 5LVD5 |
|
|
|
X | Dulcinea G. | 5DG1 | 5DG2 | 5DG3 | 5DG4 | 5DG5 |
|
|
|
X | R. Dibner |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|