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mkdir -p /gscratch/grandol1/NS5/rawdata cd /gscratch/grandol1/NS5/rawdata unpigz --to-stdout /project/microbiome/data_queue/seq/NS5/rawdata/RG_SP_500_S1_R1_001.fastq.gz | split -l 16000000 -d --suffix-length=3 --additional-suffix=.fastq - NS5_R1_ ; unpigz --to-stdout /project/microbiome/data_queue/seq/NS5/rawdata/RG_SP_500_S1_R1R2_001.fastq.gz | split -l 16000000 -d --suffix-length=3 --additional-suffix=.fastq - NS5_R2_ |
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making 240 R1 files and 240 R2 files, with structured names (e.g., for the R1 set):
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NS5_Demux.csv
is used to map MIDS to sample names and projects.
Below This Point is yet to be done
Splitting to fastq for individuals
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