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4-28-2022 Data have been returned to us. There is some confusion over the provided percent clusters passing filter in comparison with past runs, but I am proceeding with processing for nowReport confusion has been cleared up. It involved using the index reads they ran in their calculations. All samples have been pooled and were mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus normalization protocol, Normalization_Tracking_Adaptive to 10 ng/ul, leaving samples below this as is. We normalized in 20 ul, unless the starting concentration was above 100 ng/ul. We then normalized in 50 ul for those samples only. 2 ul from each sample was added to a new plate and then this plate was pooled into a column and the column was pooled into a 15 ml tube. |
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