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Samples arrived 05-05-2022

Extractions

  •  Transfer fecal material to labeled bead tubes. Weigh first few samples to approximate amount of material ~200mg
  •  Freeze dry samples
  •  Extract using MN Nucleospin 96 Stool kits following spin speeds from MN Nucleospin 96 Soil kits
  •  Transfer DNAs to plate
  •  Transfer 30 ul as working aliquot
  •  Add ISD and coligos
  •  Normalize

PCR MasterMix (28 Plates)

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