Info |
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Samples arrived 05-05-2022 |
Extractions
- Transfer fecal material to labeled bead tubes. Weigh first few samples to approximate amount of material ~200mg
- Freeze dry samples
- Extract using MN Nucleospin 96 Stool kits following spin speeds from MN Nucleospin 96 Soil kits
- Transfer DNAs to plate
- Transfer 30 ul as working aliquot
- Add ISD and coligos
- Normalize
PCR MasterMix (28 Plates)
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