| Info |
|---|
Plates in red have been extracted, PCRed, cleaned, quantified, and normalized by 7-15-22. Bolded red have been qPCRed. |
NS6 Plates
6AC1 | 6AC2 | 6AC3 | |
6BM1 | 6BM2 | ||
6LVD1 | 6LVD2 | 6LVD3 | 6LVD4 |
6RD1 | 6RD2 | 6RD3 | |
6GTL1 | 6GTL2 | ||
6RD4 | 6RD5 | 6RD6 | 6RD7 |
6LVD5 | 6LVD6 | ||
MP002 | MP003 | MP006 |
...
MasterMix (make for 16 plates in 50mL conical tube)
...
Add 11 ul to each well of a hard shell, full skirt plate. Seal with bubble strips and store in refrigerator until needed label “NS5 “NS6 PCR”. (If doing manually, one needs 2 ul of template and 2 ul of the primers).
...
Plate | 16S Primer | ITS Primer |
|---|---|---|
6LVD4 | 16S0A3 | ITS0A3 |
16S0B3 | ITS0B3 | |
6RD1 | 16S0C3 | ITS0C3 |
16S0D3 | ITS0D3 | |
6RD2 | 16S0E3 | ITS0E3 |
16S0F3 | ITS0F3 | |
6NW16RD3 | 16S0G3 | ITS0G3 |
16S0H3 | ITS0H3 |
Run on Thermocycler Program GSAF36:
...
Plate | 16S Primer | ITS Primer | 6NW2|
|---|---|---|---|
16S0A4 | ITS0A4 | ||
16S0B4 | ITS0B4 | ||
6NW3 | 16S0C4 | ITS0C4 | |
16S0D4 | ITS0D4 | 6NW4||
16S0E4 | ITS0E4 | ||
16S0F4 | ITS0F4 | ||
6GTL1 | 16S0G4 | ITS0G4 | |
16S0H4 | ITS0H4 | ||
6GTL2 | 16S0A5 | ITS0A5 | |
16S0B5 | ITS0B5 |
...