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Dilute 1 nM full pool to loading concentration of 60 pM:
Add 6 ul 1 nM Pool to 84 82 ul “10 mM Tris 8.5” and 10 12 ul 50 pM PhiX
Remove iSeq 100 i1 Flow Cell from refrigerator 5’s crisper drawer and open white foil pack and allow to equilibrate to RT for 10-15 minutes.
Open “iSeq 100 i1 Reagent Cartridge v2”. Turn on iSeq100
Click on “Sequence”. Watch Video. Do what video tells you to do. Follow on screen instructions until run starts.
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