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ul/rxn | Reagent | # of rxns | ul needed | |
|---|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 450 | 1350 | 600 |
0.45 | 10M dNTPs | 450 | 202.5 | 90 |
0.3 | Kapa HiFi HotStart DNA Pol | 450 | 135 | 60 |
8.25 | HPLC H2O | 450 | 3713 | 1650 |
12 | Total Volume | 450 | 5400 | 2400 |
Plate | TRNL Primers | fullITS Primers |
|---|---|---|
2TRNL1 | TRNL19 | fullITS05 |
TRNL20 | fullITS06 | |
2TRNL2 | TRNL17 | fullITS07 |
TRNL18 | fullITS08 | |
2TRNL3 | TRNL21 | fullITS09 |
TRNL22 | fullITS10 | |
2TRNL4 | TRNL23 | fullITS11 |
TRNL24 | fullITS12 | |
2TRNL5 | TRNLTRNL16 | fullITSfullITS02 |
Template Format:
Run TRNL plates on thermocycler program TRNL_T*:
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