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Cleanup each full pool via ultra purification
Size select for 250-350bp fragments size window via Pippin Prep
Sending Out for Sequencing to Admera
5% PhiX spike
Library 1
SAR_Plate1_Lib1
SAR_Plate3_Lib1
SAR_Plate5_Lib1
SAR_Plate7_Lib1
SAR_Plate9_Lib1
SAR_Plate11_Lib1
SAR_Under20_Lib1
Library 2
SAR_Plate2_Lib2
SAR_Plate4_Lib2
SAR_Plate6_Lib2
SAR_Plate8_Lib2
SAR_Plate10_Lib2
SAR_Plate12_Lib2
SAR_Over750_Lib2
Check In Samples Against List from Sam Patrick Johnson
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Dilute Super high tubes an additional 3x by adding 60 ul TE
High plate Plate 12 was diluted 4x by adding 60 ul TE; plate 3x by adding 40ul
Both plates and tubes were requantified
Normalize plates with TE on Nimbus
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GTL Plate | EcoR1 MID plate | Pool Plate | Library | Norm to | Sequencer |
|---|---|---|---|---|---|
1Sauger1 | 1 | ||||
1Sauger3 | 2 | ||||
1Sauger5 | 3 | ||||
1Sauger7 | 4 | ||||
1Sauger9 | 5 | ||||
1Sauger11 | 6 | ||||
1Sauger2 | 1 | ||||
1Sauger4 | 2 | ||||
1Sauger6 | 3 | ||||
1Sauger8 | 4 | ||||
1Sauger10 | 5 | ||||
1Sauger12 | 6 | ||||
1SaugerUnderOver | 7 | ||||
Label reaction plates with MID plate used.
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