Page Comparison

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	1	2	3	4	5	6	7
A	NTC	NTC	NTC	PE1 A1	JC1 A1	PE1 A1 Take 2	JC1 A1 Take2
B	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std	PE1 B1	JC1 B1	PE1 B1 Take 2	JC1 B1 Take 2
C	0.002 pM Std	0.002 pM Std	0.002 pM Std	PE1 C1	JC1 C1	PE1 C1 Take 2	JC1 C1 Take 2
D	0.02 pM Std	0.02 pM Std	0.02 pM Std	PE1 D1	JC1 D1	PE1 D1 Take 2	JC1 D1 Take 2
E	0.2 pM Std	0.2 pM Std	0.2 pM Std	PE1 E1	JC1 E1	PE1 E1 Take 2	JC1 E1 Take 2
F	2 pM Std	2 pM Std	2 pM Std	PE1 F1	JC1 F1	PE1 F1 Take 2	JC1 F1 Take 2
G	20 pM Std	20 pM Std	20 pM Std	PE1 G1	JC1 G1	PE1 G1 Take 2	JC1 G1 Take 2
H				PE1 H1	JC1 H1	PE1 H1 Take 2	JC1 H1 Take 2

Well, there is certainly a difference between the initial PCR2 and the redone version. There is a 100 fold increase in the redone version.

View file

name	NovaSeq2_PCR2redos.csv

We will repeat this one more time with the flow cell primers created anew from the 300 uM stock using one plate, PE1. We will also do a repeated PCR1 for this plate to include in the qPCR comparison.

Versions Compared

Old Version 2

New Version 3

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