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We will repeat this one more time with the flow cell primers created anew from the 300 uM stock using one plate, PE1. We will also do a repeated PCR1 for this plate to include in the qPCR comparison.
1:1000 dilutions of final PCRs loaded.
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | NTC | NTC | NTC | PE1 A1 Take 1 | PE1 A1 Take 2 | PE1 A1 Take 3 | PE1 A1 Take 4 | |||||
B | 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std | PE1 B1 Take 1 | PE1 B1 Take 2 | PE1 B1 Take 3 | PE1 B1 Take 4 | |||||
C | 0.002 pM Std | 0.002 pM Std | 0.002 pM Std | PE1 C1 Take 1 | PE1 C1 Take 2 | PE1 C1 Take 3 | PE1 C1 Take 4 | |||||
D | 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | PE1 D1 Take 1 | PE1 D1 Take 2 | PE1 D1 Take 3 | PE1 D1 Take 4 | |||||
E | 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | PE1 E1 Take 1 | PE1 E1 Take 2 | PE1 E1 Take 3 | PE1 E1 Take 4 | |||||
F | 2 pM Std | 2 pM Std | 2 pM Std | PE1 F1 Take 1 | PE1 F1 Take 2 | PE1 F1 Take 3 | PE1 F1 Take 4 | |||||
G | 20 pM Std | 20 pM Std | 20 pM Std | PE1 G1 Take 1 | PE1 G1 Take 2 | PE1 G1 Take 3 | PE1 G1 Take 4 | |||||
H | PE1 H1 Take 1 | PE1 H1 Take 2 | PE1 H1 Take 3 | PE1 H1 Take 4 |
Take 1: Original Prep
Take2: PCR2 redone from original PCR1 with original flow cell primer dilutions
Take3: PCR2 redone from original PCR1 with new dilutions of flow cell primers from original 300 uM stock
Take 4: Fresh full prep using new dilutions of flow cell primers and original normalized template plate