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Keep MasterMix in the dark as much as possible. The qPCR dyes are light sensitive and degrade with exposure.
Add MasterMix and Samples:
Add 16 ul of Mastermix to each well needed.
Add 4 ul of each sample.
Setup Instrument For Run
Open “7500 System Software”
Hit New File Icon
Name Plate Appropriately (Copy from Confluence Page in Question). Hit “Next”.
Select “Unknown”. Hit “Add”. Click “Next”
Highlight All Wells To Be Used And Assign “Unknown” Detector
Assign All Wells Their Task From The Dropdown next to “Unknown” detector. Choices Are “NTC”, “Unknown”, or “Standard”. Click “Finish”
Assign Standards Values:
Std1: 20, Std2: 2, Std3: 0.2, Std4: 0.02, Std5: 0.002, Std6: 0.0002
Navigate to “Instrument” tab and change cycle parameters to:
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