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4PA1_PCR1_16S0A1, 4PA1_PCR1_16S0B1, 4PA1_PCR1_ITS0A1, and 4PA1_PCR1_ITS0B1
When these plates are combined for the first cleanup, the two MIDplateCodes are appended and the PCR2 plate continues with the same name except PCR2 is used instead of PCR1.
Cleaned Plates Being Stored: 4PA1_PCR1_16S0A1_16S0B1 , and 4PA1_PCR1_ITS0A1_ITS0B1
Plates proceeding with PCR2: 4PA1_PCR2_16S0A1_16S0B1 , and 4PA1_PCR2_ITS0A1_ITS0B1
Only one plate should be saved per HTX plate reader file; hit “end of batch” after reading absorbances for the second half of the plate. The quantification of the template plus controls should be saved on the petalibrary under MicroLab/DnaQuantification/NovaSeq4/PCR2/. The above mentioned plates would be 4PA1_PCR2_16S.xlsx and 4PA1_PCR2_ITS.xlsx
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